Description

2'-o-tert-Butyldimethylsilyl-5'-o-Dmt-3-Methyluridine 3'-Ce Phosphoramidite is a high-purity, protected nucleoside phosphoramidite monomer, a critical building block for the solid-phase synthesis of oligonucleotides. This compound is essential for researchers and manufacturers requiring precise control over nucleic acid sequence and modification, enabling the development of advanced therapeutics, diagnostics, and research tools. It is primarily used by biotechnology and pharmaceutical companies engaged in the production of antisense oligonucleotides, siRNA, aptamers, and other synthetic nucleic acid constructs.

Application

• Solid-Phase Oligonucleotide Synthesis: As a key monomer for the automated synthesis of modified RNA strands.
• Therapeutic Oligonucleotide Manufacturing: Production of active pharmaceutical ingredients (APIs) for antisense, siRNA, and miRNA-based drugs.
• Diagnostic Probe Development: Synthesis of labeled or modified probes for genomic detection and analysis.
• Research-Grade Oligo Synthesis: For academic and industrial research into nucleic acid structure, function, and interactions.
• Aptamer Production: Building block for creating libraries of synthetic nucleic acid aptamers for target binding.
• Introduction of Specific Modifications: Enables the site-specific incorporation of a 3-methyluridine modification into an oligonucleotide chain to study or enhance stability and binding properties.

Basic Information

Product Name	2'-o-tert-Butyldimethylsilyl-5'-o-Dmt-3-Methyluridine 3'-Ce Phosphoramidite
CAS No.	179762-50-6
Molecular Formula	C46H63N4O9PSi
Molecular Weight	875.07 g/mol
Synonyms	3'-[(2-Cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite of 5'-O-(4,4'-dimethoxytrityl)-2'-O-(tert-butyldimethylsilyl)-3-methyluridine; 2'-O-TBDMS-5'-O-DMT-3-methyluridine-3'-CE phosphoramidite; 3-Methyluridine, 5'-O-[bis(4-methoxyphenyl)phenylmethyl]-2'-O-[(1,1-dimethylethyl)dimethylsilyl]-, 3'-[2-cyanoethyl bis(1-methylethyl)phosphoramidite]; DMT-2'-O-TBDMS-3-Me-U-CE Phosphoramidite; 3-Methyl-5'-O-DMT-2'-O-TBDMS-uridine 3'-CE phosphoramidite; 3-Me-U Phosphoramidite (2'-O-TBDMS, 5'-O-DMT).
EINECS	Contact for details

Quality Control

Our 2'-o-tert-Butyldimethylsilyl-5'-o-Dmt-3-Methyluridine 3'-Ce Phosphoramidite is manufactured under strict quality systems to ensure consistency and reliability for critical oligonucleotide synthesis. Each batch is subjected to rigorous analytical testing, including HPLC purity analysis, 31P and 1H NMR spectroscopy for structural confirmation, and moisture content determination. Certificates of Analysis (COA) detailing all specifications are provided to guarantee the material meets the high standards required for pharmaceutical development and research applications.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere (argon or nitrogen) in a dry, moisture-free environment. This product is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions to maintain stability and reactivity.

Specification

Item	Specification
Appearance	White to off-white solid or foam
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 97.0%
Water Content (KF)	≤ 0.5%
Solubility	Soluble in anhydrous acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.