Description

2-Isobutyrylamino-9-(2'-o-tert-Butyldimethylsilyl-5'-o-Dmt-B-D-Ribofuranosyl)Purine 3'-Ce Phosphoramidite is a high-purity, protected phosphoramidite monomer specifically designed for the solid-phase synthesis of oligonucleotides. This compound is critical for ensuring high coupling efficiency and sequence fidelity in the automated production of DNA and RNA strands. It is an essential raw material for research institutions, diagnostic kit manufacturers, and pharmaceutical companies engaged in the development of antisense oligonucleotides, siRNAs, aptamers, and other therapeutic nucleic acids.

Application

• Solid-Phase Oligonucleotide Synthesis: As a key building block (phosphoramidite) for the automated synthesis of DNA and RNA sequences.
• Therapeutic Nucleic Acid R&D: Production of antisense oligonucleotides (ASOs), small interfering RNA (siRNA), and aptamers for drug discovery and development.
• Diagnostic Probe Manufacturing: Synthesis of labeled probes and primers used in PCR, qPCR, sequencing, and microarray technologies.
• Research-Grade Oligo Production: For academic and industrial laboratories requiring custom oligonucleotides for genetic research and molecular biology.
• Synthesis of Modified Oligonucleotides: Enabling the incorporation of specific purine modifications to alter stability, binding affinity, or functionality.

Basic Information

Product Name	2-Isobutyrylamino-9-(2'-o-tert-Butyldimethylsilyl-5'-o-Dmt-B-D-Ribofuranosyl)Purine 3'-Ce Phosphoramidite
CAS No.	179558-91-9
Molecular Formula	C46H61N6O7PSi
Molecular Weight	869.07 g/mol
Synonyms	2-Isobutyrylamino-9-(2'-O-TBDMS-5'-O-DMT-β-D-ribofuranosyl)purine 3'-CE Phosphoramidite; 2-Isobutyryl-G Phosphoramidite (2'-O-TBDMS, 5'-O-DMT); 2-Ibu-G CE Phosphoramidite; 9-[(2R,3R,4R,5R)-5-[[Bis(4-methoxyphenyl)-phenylmethoxy]methyl]-4-[(2-cyanoethoxy)-diisopropylaminophosphanyl]oxy-3-[(2-methylpropanoyl)amino]-2-[(tert-butyldimethylsilyl)oxy]oxolan-2-yl]-6-(diaminomethylideneamino)purin-1-ium; DMT-2'-O-TBDMS-2-Isobutyryl-Guanosine-3'-CE Phosphoramidite; Guanosine, 2'-O-[(1,1-dimethylethyl)dimethylsilyl]-N-isobutyryl-5'-O-(4,4'-dimethoxytrityl)-, 3'-[2-cyanoethyl N,N-diisopropylphosphoramidite]
EINECS	Contact for details

Quality Control

Our phosphoramidite monomers are produced under strict quality control protocols in a controlled environment to ensure batch-to-batch consistency and high purity. Each lot is thoroughly characterized by HPLC, NMR (¹H and ³¹P), and mass spectrometry to confirm identity, purity, and stability. A comprehensive Certificate of Analysis (COA) detailing all specifications is provided with every shipment to support your quality assurance and regulatory needs.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere (argon or nitrogen) in a desiccated environment. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the sealed vial to equilibrate to room temperature before opening to prevent moisture condensation.

Specification

Item	Specification
Appearance	White to off-white solid or foam
Identification (HPLC)	Conforms to reference standard
Identification (³¹P NMR)	Conforms to reference standard
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 0.5%
Solubility	Clear, colorless solution in anhydrous acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.