Description

Phosphoramidous Acid, N,n-Bis(1-methylethyl)-, 2-cyanoethyl 12-[[(4-methoxyphenyl)diphenylmethyl]amino]dodecyl ester is a sophisticated, high-purity phosphoramidite monomer, a critical building block in automated oligonucleotide synthesis. This compound matters for its role in producing high-fidelity DNA and RNA sequences, enabling advanced research and therapeutic development. It is essential for biotechnology, pharmaceutical, and diagnostic companies engaged in antisense therapy, gene editing, and molecular diagnostics.

Application

• Solid-Phase Oligonucleotide Synthesis: As a key phosphoramidite reagent for the controlled, stepwise addition of nucleosides in DNA/RNA synthesizers.
• Therapeutic Oligonucleotide Production: Manufacturing of antisense oligonucleotides (ASOs), small interfering RNA (siRNA), and aptamers for targeted therapies.
• Research-Grade Primer & Probe Synthesis: Production of custom primers, probes, and gene fragments for PCR, sequencing, and genomic research.
• Diagnostic Kit Manufacturing: Creation of labeled probes and capture sequences for in vitro diagnostic (IVD) assays and biosensors.
• Development of Modified Oligonucleotides: Synthesis of oligonucleotides with specific backbone or sugar modifications for enhanced stability and specificity.

Basic Information

Product Name	Phosphoramidous Acid, N,n-Bis(1-methylethyl)-, 2-cyanoethyl 12-[[(4-methoxyphenyl)diphenylmethyl]amino]dodecyl Ester
CAS No.	178925-51-4
Molecular Formula	C46H62N3O4P
Molecular Weight	751.98 g/mol
Synonyms	DMT-C12-Amino-Modifier Phosphoramidite; 12-[(4-Methoxytriphenylmethyl)amino]dodecyl-1-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite; C12 Amino Modifier Phosphoramidite; Amino-Modifier C12 Phosphoramidite; 5'-Dimethoxytrityl-12-amino-dodecyl-1-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite; 12-Amino-dodecyl phosphoramidite; DMT-C12-NH2 Phosphoramidite
EINECS	Contact for details

Quality Control

Our Phosphoramidous Acid, N,n-Bis(1-methylethyl)-, 2-cyanoethyl 12-[[(4-methoxyphenyl)diphenylmethyl]amino]dodecyl ester is manufactured under strict quality systems to ensure the high purity and consistency required for sensitive oligonucleotide synthesis. Each batch is supported by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and moisture content. We adhere to industry best practices and can provide material tailored to meet specific research or cGMP guidelines upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere (argon or nitrogen) in a dry, moisture-free environment. This compound is hygroscopic (moisture-sensitive) and easily oxidized; prolonged exposure to air, light, or ambient humidity will compromise stability and reactivity. Allow the sealed container to equilibrate to room temperature before opening to prevent condensation.

Specification

Item	Specification
Appearance	White to off-white solid or foam
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 97.0%
Water Content (KF)	≤ 0.5%
Solubility	Soluble in anhydrous acetonitrile, dichloromethane

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.