Description

5’-Dmtr-Dc (Ac)-Methylphosphonamidite is a high-purity phosphoramidite monomer, a critical building block for the solid-phase synthesis of oligonucleotides. Its primary value lies in enabling the precise and efficient incorporation of modified or standard nucleosides into DNA and RNA sequences. This compound is essential for researchers and manufacturers in the fields of therapeutic oligonucleotide development, molecular diagnostics, and genetic research, where reliable synthesis of high-quality probes and primers is paramount.

Application

• Oligonucleotide Synthesis: As a key phosphoramidite reagent for the automated solid-phase synthesis of DNA and RNA strands.
• Antisense & siRNA Therapeutics: Production of chemically modified oligonucleotides for gene silencing and therapeutic applications.
• Molecular Probes & Primers: Manufacture of labeled or unlabeled probes for PCR, sequencing, and hybridization assays.
• Diagnostic Kit Manufacturing: Synthesis of specific oligonucleotide components used in diagnostic tests and kits.
• Research & Development: Academic and industrial R&D for studying nucleic acid chemistry, structure, and function.
• Aptamer Development: Construction of synthetic DNA/RNA libraries for the selection of high-affinity binding molecules.

Basic Information

Product Name	5’-Dmtr-Dc (Ac)-Methylphosphonamidite
CAS No.	178094-78-5
Molecular Formula	C43H49N4O9P
Molecular Weight	820.85 g/mol
Synonyms	5'-O-(4,4'-Dimethoxytrityl)-N4-acetyl-2'-deoxycytidine-3'-O-methyl N,N-diisopropylphosphoramidite; DMT-dC(Ac)-CE Phosphoramidite; N4-Acetyl-5'-O-(4,4'-dimethoxytrityl)-2'-deoxycytidine-3'-O-(2-cyanoethyl-N,N-diisopropyl)phosphoramidite (common trade form); dC(Ac) Phosphoramidite; Ac-dC Phosphoramidite; 2'-Deoxycytidine, N4-acetyl-5'-O-[bis(4-methoxyphenyl)phenylmethyl]-, 3'-[2-cyanoethyl bis(1-methylethyl)phosphoramidite]; DNA Synthesis Reagent; Cytidine Phosphoramidite (protected)
EINECS	Contact for details

Quality Control

Every batch of 5’-Dmtr-Dc (Ac)-Methylphosphonamidite is manufactured under strict quality systems and undergoes comprehensive analytical testing to ensure it meets the stringent requirements for oligonucleotide synthesis. We provide a detailed Certificate of Analysis (COA) with each shipment, reporting key parameters such as purity, moisture content, and functional performance. Our quality commitment aligns with the standards expected for GMP-grade raw materials used in therapeutic and diagnostic applications.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere (argon or nitrogen) in a desiccated environment. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the sealed container to equilibrate to room temperature before opening to prevent condensation and moisture ingress.

Specification

Item	Specification
Appearance	White to off-white solid or foam
Identification (HPLC)	Conforms to reference standard
Purity (HPLC)	≥ 98.5%
Water Content (KF)	≤ 0.5%
Solubility	Soluble in anhydrous acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.