Description

5'-o-Dmt-2'-o-Propynyluridine 3'-Ce Phosphoramidite is a high-purity, chemically modified nucleoside phosphoramidite building block essential for the automated synthesis of oligonucleotides. Its core value lies in enabling the precise incorporation of 2'-O-propargyl modifications into RNA sequences, a critical step for advanced therapeutic and diagnostic applications. This product is primarily needed by research institutions and biotechnology companies engaged in the development of antisense oligonucleotides, siRNA, and other next-generation nucleic acid therapeutics.

Application

• Solid-Phase Oligonucleotide Synthesis: As a key monomer for the automated synthesis of modified RNA strands on DNA/RNA synthesizers.
• Therapeutic Oligonucleotide Development: For creating antisense oligonucleotides (ASOs) and small interfering RNA (siRNA) with enhanced stability and pharmacokinetic properties.
• Click Chemistry Conjugation: The 2'-O-propargyl group serves as a handle for post-synthetic functionalization via copper-catalyzed azide-alkyne cycloaddition (CuAAC) to attach labels, dyes, or targeting ligands.
• Structural Biology Studies: Used to synthesize probes for investigating RNA structure, function, and protein-RNA interactions.
• Diagnostic Probe Synthesis: Manufacturing of high-specificity probes for molecular diagnostics, including FISH and PCR applications.
• Basic Research: A vital reagent in academic and industrial labs exploring the frontiers of nucleic acid chemistry and biology.

Basic Information

Product Name	5'-o-Dmt-2'-o-Propynyluridine 3'-Ce Phosphoramidite
CAS No.	171486-62-7
Molecular Formula	C46H51N4O9P
Molecular Weight	834.90 g/mol
Synonyms	5'-O-(4,4'-Dimethoxytrityl)-2'-O-propargyluridine 3'-(2-cyanoethyl N,N-diisopropylphosphoramidite); 2'-O-Propargyl-5'-O-DMT-uridine 3'-CE Phosphoramidite; DMT-2'-O-Propargyluridine-CE Phosphoramidite; 2'-O-Propargyl-Uridine Phosphoramidite; Uridine, 5'-O-[bis(4-methoxyphenyl)phenylmethyl]-2'-O-(2-propyn-1-yl)-, 3'-(2-cyanoethyl N,N-diisopropylphosphoramidite)
EINECS	Contact for details

Quality Control

Every batch of 5'-o-Dmt-2'-o-Propynyluridine 3'-Ce Phosphoramidite is manufactured and tested under strict quality systems to ensure consistency and reliability for critical synthesis workflows. Our products undergo rigorous quality testing, including HPLC for purity and 31P NMR for identity and stability, to ensure compliance with industry standards for nucleic acid research. Certificates of Analysis (COA) with detailed chromatographic data are provided with each shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C ± 5°C under an inert atmosphere (argon or nitrogen) in a dry, moisture-free environment. Due to its hygroscopic nature, the product should be brought to room temperature in a desiccator before opening to prevent condensation and hydrolysis.

Specification

Item	Specification
Appearance	White to off-white solid or foam
Identification (HPLC)	Conforms to reference standard
Identification (31P NMR)	Conforms to reference standard
Purity (HPLC)	≥ 97.0%
Moisture (Karl Fischer)	≤ 1.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.