Description

N2-iso-Butyroyl-5'-o-(4,4'-Dimethoxytrityl)-2'-o-Propargyl Guanosine-3'-o-[(2-Cyanoethyl)-(N,n-Diisopropyl)]Phosphoramidite is a high-purity, modified phosphoramidite monomer essential for the solid-phase synthesis of oligonucleotides. It matters because it enables the precise incorporation of a propargyl-protected guanosine residue, a critical building block for advanced nucleic acid research and therapeutic development. This product is specifically needed by pharmaceutical R&D teams, diagnostic developers, and academic researchers working on antisense oligonucleotides, siRNA, aptamers, and other novel genetic tools.

Application

• Solid-Phase Oligonucleotide Synthesis: As a key phosphoramidite building block in automated DNA/RNA synthesizers.
• Antisense Oligonucleotide (ASO) Development: For the synthesis of research and therapeutic-grade oligonucleotides targeting specific mRNA sequences.
• siRNA and miRNA Synthesis: Enabling the production of short interfering RNA and microRNA strands for gene silencing studies.
• Aptamer Production: Used in creating structured nucleic acid libraries for selection of high-affinity binding molecules.
• Probe and Primer Synthesis: Manufacturing labeled or modified oligonucleotides for diagnostic assays and PCR applications.
• Structural Studies: Incorporating modified bases to study nucleic acid structure, function, and protein interactions.
• Therapeutic Candidate Screening: Supplying GMP-grade starting material for pre-clinical and clinical-stage oligonucleotide drug manufacturing.

Basic Information

Product Name	N2-iso-Butyroyl-5'-o-(4,4'-Dimethoxytrityl)-2'-o-Propargyl Guanosine-3'-o-[(2-Cyanoethyl)-(N,n-Diisopropyl)]Phosphoramidite
CAS No.	171486-61-6
Molecular Formula	C52H60N7O9P
Molecular Weight	958.06 g/mol
Synonyms	2'-O-Propargyl-G(Ibu) Phosphoramidite; 5'-O-DMT-2'-O-propargylguanosine, 3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite, N2-Isobutyryl; Guanosine, N2-(2-methyl-1-oxopropyl)-5'-O-[bis(4-methoxyphenyl)phenylmethyl]-2'-O-2-propyn-1-yl-3'-O-[2-cyanoethyl bis(1-methylethyl)phosphoramidite]; DMT-2'-O-Propargyl-G(Ibu)-CE Phosphoramidite; 2'-O-Alkynyl Guanosine Phosphoramidite.
EINECS	Contact for details

Quality Control

Our N2-iso-Butyroyl-5'-o-(4,4'-Dimethoxytrityl)-2'-o-Propargyl Guanosine-3'-o-[(2-Cyanoethyl)-(N,n-Diisopropyl)]Phosphoramidite is manufactured under strict quality systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation by NMR and MS, to ensure it meets the stringent requirements for oligonucleotide synthesis. Certificates of Analysis (COA) with full traceability are provided. We support development with R&D and GMP-grade materials, adhering to relevant ICH guidelines.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C ± 5°C under an inert atmosphere (argon or nitrogen) in a dry, moisture-free environment. This product is hygroscopic (moisture-sensitive) and easily oxidized. Allow the sealed vial to equilibrate to room temperature before opening to prevent condensation. For long-term storage, keep desiccated.

Specification

Item	Specification
Appearance	White to off-white solid or foam
Identification (HPLC)	Retention time corresponds to reference standard
Identification (NMR)	Spectrum consistent with structure
Purity (HPLC)	≥ 97.0%
Water Content (KF)	≤ 1.0%
Solubility	Clear, colorless solution in anhydrous acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.