Description

Phosphoramidous Acid, Bis(1-Methylethyl)-, 16,16-Bis(4-Methoxyphenyl)-16-Phenyl-3,6,9,12,15-Pentaoxahexadec-1-Yl 2-Cyanoethyl Ester is a sophisticated, high-purity phosphoramidite monomer, a critical building block in automated oligonucleotide synthesis. This compound is engineered for the precise and efficient introduction of modified nucleosides into DNA and RNA sequences, enabling advanced research and therapeutic development. It is essential for scientists and manufacturers in the fields of pharmaceuticals, molecular diagnostics, and biotechnology who require reliable, high-quality reagents for antisense oligonucleotides, siRNA, and other nucleic acid-based applications.

Application

• Solid-Phase Oligonucleotide Synthesis: A key phosphoramidite reagent for the automated synthesis of DNA and RNA strands on solid support.
• Therapeutic Oligonucleotide Manufacturing: Used in the production of antisense drugs, siRNA, and aptamers for targeted therapies.
• Molecular Probe Development: Essential for creating labeled or modified probes used in diagnostics, FISH (Fluorescence In Situ Hybridization), and genomic research.
• Research-Grade Oligo Synthesis: Enables academic and industrial research labs to synthesize custom oligonucleotides with specific modifications.
• Next-Generation Sequencing (NGS): Used in the preparation of specialized oligonucleotide libraries and adapters.
• PCR and qPCR Assay Development: For manufacturing primers and probes with high specificity and performance.

Basic Information

Product Name	Phosphoramidous Acid, Bis(1-Methylethyl)-, 16,16-Bis(4-Methoxyphenyl)-16-Phenyl-3,6,9,12,15-Pentaoxahexadec-1-Yl 2-Cyanoethyl Ester
CAS No.	165682-44-0
Molecular Formula	C44H55N2O9P
Molecular Weight	786.90 g/mol
Synonyms	DMTr-PEG-Phosphoramidite; DMT-PEG-Phosphoramidite; Dimethoxytrityl-PEG-Phosphoramidite; 1-[Bis(4-methoxyphenyl)phenylmethoxy]-3,6,9,12,15-pentaoxaheptadecan-17-yl 2-cyanoethyl diisopropylphosphoramidite; DMT-PEG5-Phosphoramidite; 5k DMT-PEG Phosphoramidite; Hydrophilic Spacer Phosphoramidite; PEGylated Phosphoramidite
EINECS	Contact for details

Quality Control

This high-value synthesis intermediate is produced under strict quality control protocols in a controlled environment. Each batch is characterized and tested to ensure it meets the stringent purity requirements for oligonucleotide synthesis. Certificates of Analysis (COA) are provided, detailing key parameters such as purity (by HPLC and 31P NMR), identity, and moisture content. Our quality system is designed to support GMP-compatible manufacturing for therapeutic applications.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere (argon or nitrogen) in a desiccated environment. This compound is strictly light-sensitive (protect from all light exposure), hygroscopic (moisture-sensitive), and easily oxidized (store under inert atmosphere). Allow the sealed container to equilibrate to room temperature before opening to prevent moisture condensation.

Specification

Item	Specification
Appearance	White to off-white solid or viscous oil
Identification (IR)	Conforms to structure
Identification (31P NMR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 0.5%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.