Description

X-Rhodamine-5'-Gtaaaacgacggccag-3'-Fluorescein CAS NO 161278-55-3 is a high-purity, dual-labeled oligonucleotide probe designed for advanced molecular diagnostics and life science research. This compound is critical for applications requiring high signal-to-noise ratios and multiplex detection capabilities due to its distinct fluorescent properties. It is primarily needed by biotechnology companies, academic research institutions, and diagnostic kit manufacturers developing sensitive assays for genetic analysis, pathogen detection, and real-time PCR.

Application

• Real-Time PCR (qPCR) Probes: Used as a hydrolysis (TaqMan) probe for the specific and quantitative detection of target DNA sequences.
• Fluorescence In Situ Hybridization (FISH): Enables high-resolution visualization and localization of specific nucleic acid sequences within cells or tissues.
• Multiplex Diagnostic Assays: Facilitates the simultaneous detection of multiple targets in a single reaction due to the spectrally distinct emissions of X-Rhodamine and Fluorescein.
• SNP Genotyping and Mutation Analysis: Provides a reliable tool for identifying single nucleotide polymorphisms and genetic mutations with high specificity.
• Pathogen Detection Kits: A key component in commercial and in-house diagnostic tests for viruses, bacteria, and other infectious agents.
• Gene Expression Profiling: Used in studies to measure and quantify the expression levels of specific genes under various conditions.

Basic Information

Product Name	X-Rhodamine-5'-Gtaaaacgacggccag-3'-Fluorescein
CAS No.	161278-55-3
Molecular Formula	C95H115N25O58P15 (Estimated for oligonucleotide sequence)
Molecular Weight	~3500 g/mol (Estimated, contact for exact value for specific synthesis lot)
Synonyms	5'-X-Rhodamine-GTAAAACGACGGCCAG-3'-Fluorescein; Dual-Labeled Oligonucleotide Probe 161278-55-3; FAM/ROX Dual-Labeled Probe; Fluorescein/X-Rhodamine Oligo Probe; TaqMan Probe Sequence; qPCR Probe; Fluorescent Oligonucleotide Conjugate; 5' ROX, 3' FAM labeled oligo
EINECS	Contact for details

Quality Control

Our X-Rhodamine-5'-Gtaaaacgacggccag-3'-Fluorescein is manufactured under strict quality systems. Each batch is characterized by HPLC for purity, Mass Spectrometry (MS) for identity and molecular weight confirmation, and UV-Vis Spectroscopy for accurate quantification and dye labeling efficiency. Certificates of Analysis (COA) detailing these specifications are provided with every shipment to ensure traceability and compliance with your research or production standards.

Storage

Preserve in a tightly closed container, protected from light. Store frozen at -20°C or below in the dark. For long-term storage, lyophilized material is recommended. The product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature before opening to prevent condensation.

Specification

Item	Specification
Appearance	Lyophilized powder or solution
Identification (HPLC/MS)	Conforms to reference standard
Purity (HPLC)	≥ 95%
Oligonucleotide Sequence	5'-GTAAAACGACGGCCAG-3'
5' Modification	X-Rhodamine (ROX)
3' Modification	Fluorescein (FAM)
Extinction Coefficient	Provided on COA
Molecular Weight	Provided on COA
Water Content (Karl Fischer)	≤ 5.0% (for lyophilized solid)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.