Description

Phosphoramidous Acid, N,n-Bis(1-methylethyl)-, 6-[Bis(4-methoxyphenyl)phenylmethoxy]hexyl 2-cyanoethyl ester is a highly specialized phosphoramidite monomer, a critical building block in automated oligonucleotide synthesis. This compound matters because it enables the precise and efficient incorporation of nucleosides into DNA and RNA strands, ensuring high coupling efficiency and yield. It is primarily needed by manufacturers and research institutions in the pharmaceutical, biotechnology, and diagnostic sectors for the production of primers, probes, antisense oligonucleotides, and other therapeutic nucleic acids.

Application

• Solid-Phase Oligonucleotide Synthesis: As a key phosphoramidite reagent in automated DNA/RNA synthesizers for the stepwise construction of oligonucleotide chains.
• Therapeutic Oligonucleotide Manufacturing: Production of antisense drugs, siRNA, and aptamers for targeted therapies.
• Diagnostic Probe Synthesis: Creation of labeled probes for PCR, microarrays, and fluorescence in situ hybridization (FISH).
• Research-Grade Oligo Production: Synthesis of primers and gene fragments for academic and industrial molecular biology research.
• Next-Generation Sequencing (NGS): Fabrication of oligonucleotide libraries and adapters used in sequencing platforms.
• CRISPR Guide RNA Synthesis: Component in the production of synthetic guide RNAs for genome editing applications.

Basic Information

Item	Details
Product Name	Phosphoramidous Acid, N,n-Bis(1-methylethyl)-, 6-[Bis(4-methoxyphenyl)phenylmethoxy]hexyl 2-cyanoethyl Ester
CAS No.	158041-82-8
Molecular Formula	C41H51N2O6P
Molecular Weight	698.83 g/mol
Synonyms	6-((Bis(4-methoxyphenyl)(phenyl)methoxy)hexyl) (2-cyanoethoxy)(diisopropylamino)phosphine; DMT-Hexyl-CE Phosphoramidite; Hexyl Spacer Phosphoramidite; 5'-Dimethoxytrityl-1',2'-Dideoxyribose-3'-[2-cyanoethyl-(N,N-diisopropyl)]-phosphoramidite (Hexyl linker); 6-(4,4'-Dimethoxytrityloxy)hexyl-1-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite; DMT-hexanol phosphoramidite; Hydrophobic Spacer Phosphoramidite C6
EINECS	Contact for details

Quality Control

Our high-purity phosphoramidite monomers are manufactured under strict quality control in a controlled environment to ensure batch-to-batch consistency for sensitive oligonucleotide synthesis. Each lot is characterized by HPLC, 31P NMR, and 1H NMR to confirm identity, purity, and stability. Certificates of Analysis (COA) with detailed chromatographic data are provided with every shipment. We adhere to ISO 9001 quality management principles, and our products are suitable for use in GMP-compliant manufacturing processes upon request.

Storage

Preserve in a tightly closed container, protected from light. Store under an inert atmosphere (argon or nitrogen) at -20°C or below in a dedicated freezer. This product is hygroscopic and easily oxidized; allow the sealed vial to equilibrate to room temperature in a desiccator before opening to prevent moisture condensation. Use anhydrous solvents and techniques for handling.

Specification

Item	Specification
Appearance	White to off-white solid or foam
Identification (HPLC)	Retention time corresponds to reference standard
Identification (NMR)	Spectra consistent with structure
Purity (HPLC)	≥ 97.0%
Water Content (KF)	≤ 0.5%
Solubility	Clear, colorless solution in anhydrous acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.