Description

2'-o-T-Butyldimethylsilyl-3'-o-[(Diisopropylamino)(2-Cyanoethoxy)Phosphino]-5'-o-(4,4'-Dimethoxytrityl)-2(1H)-Pyrimidinone-1-β-D-Riboside CAS NO 155831-90-6 is a highly specialized, phosphoramidite-protected nucleoside derivative, a critical building block in automated oligonucleotide synthesis. Its primary value lies in enabling the precise, sequential assembly of RNA and modified DNA strands for research and therapeutic applications. This compound is essential for pharmaceutical R&D and biotechnology companies developing advanced nucleic acid-based therapeutics, including antisense oligonucleotides, siRNAs, and aptamers.

Application

• Solid-Phase Oligonucleotide Synthesis: As a key phosphoramidite monomer for the automated synthesis of RNA and modified DNA sequences.
• Therapeutic Oligonucleotide Manufacturing: Production of active pharmaceutical ingredients (APIs) for siRNA, antisense, and other nucleic acid drugs.
• Research & Diagnostic Probe Development: Synthesis of labeled or modified probes for genomics, molecular diagnostics, and basic research.
• Antisense Technology: Building blocks for creating antisense oligonucleotides used in gene function studies and therapeutic candidates.
• Aptamer Synthesis: For the production of structured nucleic acid ligands used in sensing and targeted therapeutics.
• Synthesis of Modified Nucleosides: Intermediate for further chemical modification to create novel nucleoside analogs.

Basic Information

Product Name	2'-o-T-Butyldimethylsilyl-3'-o-[(Diisopropylamino)(2-Cyanoethoxy)Phosphino]-5'-o-(4,4'-Dimethoxytrityl)-2(1H)-Pyrimidinone-1-β-D-Riboside
CAS No.	155831-90-6
Molecular Formula	C52H67N4O9PSi
Molecular Weight	951.17 g/mol
Synonyms	5'-O-(4,4'-Dimethoxytrityl)-2'-O-(tert-butyldimethylsilyl)-3'-O-[(diisopropylamino)(2-cyanoethoxy)phosphino]uridine; Uridine, 5'-O-[bis(4-methoxyphenyl)phenylmethyl]-2'-O-[(1,1-dimethylethyl)dimethylsilyl]-3'-O-[bis(1-methylethyl)aminophosphino]-; 2'-O-TBDMS-3'-O-(2-cyanoethyl-N,N-diisopropyl)phosphoramidite-5'-O-DMT-uridine; DMT-2'-O-TBDMS-Uridine Phosphoramidite; Uridine Phosphoramidite (2'-O-TBDMS, 5'-O-DMT); RNA Phosphoramidite Monomer (U); 2'-O-TBDMS-rU Phosphoramidite.
EINECS	Contact for details

Quality Control

This high-value synthetic intermediate is produced under strict quality control protocols suitable for pharmaceutical development. Every batch is characterized by advanced analytical techniques including HPLC, 31P NMR, and 1H NMR to ensure identity, purity, and reactivity. Our quality system is designed to support regulatory filings, and a comprehensive Certificate of Analysis (COA) detailing purity, moisture content, and solvent residues is provided with each shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere (argon or nitrogen) in a desiccated environment. This compound is hygroscopic (moisture-sensitive) and light-sensitive. Allow the sealed vial to equilibrate to room temperature before opening to prevent condensation and moisture ingress.

Specification

Item	Specification
Appearance	White to off-white solid or foam
Identification (HPLC)	Conforms
Identification (NMR)	Conforms
Purity (HPLC)	≥ 97.0%
Water Content (KF)	≤ 1.0%
Solvent Residues (GC)	Meets ICH guidelines
Phosphoramidite Content (31P NMR)	≥ 95.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.