Description

6-Carboxytetramethylrhodamine Succinimidyl Ester is a high-purity, amine-reactive fluorescent labeling reagent widely used in the preparation of biological probes. Its core value lies in enabling the covalent conjugation of the bright, photostable TAMRA fluorophore to proteins, antibodies, and other biomolecules for sensitive detection and imaging. This compound is essential for researchers and manufacturers in molecular diagnostics, flow cytometry, fluorescence microscopy, and immunoassay development who require reliable, high-performance labeling.

Application

• Fluorescent Labeling of Proteins and Antibodies: Conjugation to primary amines (lysine residues) for creating detection reagents in immunoassays (ELISA, Western Blot) and immunohistochemistry.
• Nucleic Acid Probe Synthesis: Labeling of oligonucleotides (e.g., primers, probes) for fluorescence in situ hybridization (FISH) and real-time PCR applications.
• Cell Tracing and Tracking: Preparation of fluorescent dyes for long-term cell labeling and tracking in flow cytometry and live-cell imaging studies.
• Bioconjugation and Chemical Biology: A key building block for creating custom fluorescent conjugates of peptides, enzymes, and other biomolecules for research and diagnostic kits.
• Microarray and Biochip Fabrication: Used to label biomolecules immobilized on solid surfaces for high-throughput screening and multiplexed detection platforms.
• Pharmaceutical Analysis: Derivatization agent for the sensitive HPLC or LC-MS analysis of amine-containing drugs and metabolites.

Basic Information

Product Name	6-Carboxytetramethylrhodamine Succinimidyl Ester
CAS No.	150810-69-8
Molecular Formula	C32H31N3O7
Molecular Weight	569.61 g/mol
Synonyms	6-TAMRA, SE; 6-Carboxy-tetramethylrhodamine N-succinimidyl ester; 5(6)-TAMRA, SE; 6-TAMRA NHS ester; 6-Carboxytetramethylrhodamine, succinimidyl ester; TAMRA 6-isomer NHS ester; 6-TMR NHS ester; X-rhodamine-6-carboxylic acid, succinimidyl ester
EINECS	Contact for details

Quality Control

Our 6-Carboxytetramethylrhodamine Succinimidyl Ester is manufactured under strict quality systems to ensure batch-to-batch consistency and high performance in sensitive applications. Each lot undergoes rigorous analytical testing, including HPLC for purity and assay, spectroscopic identification (UV-Vis, IR), and moisture content analysis. Certificates of Analysis (COA) detailing all specifications are provided with every shipment to support your quality assurance and regulatory documentation.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake. For long-term stability, store under inert atmosphere.

Specification

Item	Specification
Appearance	Dark red to purple solid
Identification (HPLC)	Conforms
Identification (IR)	Conforms
Purity (HPLC)	≥ 95.0%
Absorption (λmax)	542 - 552 nm
Emission (λmax)	565 - 575 nm
Water Content (KF)	≤ 2.0%
Solubility	Soluble in DMF, DMSO, acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.