Description

N(4)-Methyl-2'-Deoxycytidine 5'-Triphosphate is a chemically modified nucleotide triphosphate analog crucial for advanced molecular biology and biochemistry research. This compound matters as it serves as a substrate for DNA polymerases, enabling the site-specific incorporation of epigenetic markers or modified bases into DNA strands, which is essential for studying DNA-protein interactions, replication, and epigenetic mechanisms. It is primarily needed by researchers and manufacturers in the fields of next-generation sequencing (NGS), PCR applications, synthetic biology, and pharmaceutical development for creating probes and functional nucleic acids.

Application

• Next-Generation Sequencing (NGS): Used as a modified dNTP for sequencing-by-synthesis platforms to study DNA methylation and epigenetic patterns.
• PCR and qPCR: Employed in the synthesis of specifically modified DNA primers and probes for enhanced detection and analysis.
• Synthetic Biology: A key building block for the enzymatic synthesis of DNA containing site-specific modifications for novel genetic circuits and biosensors.
• Epigenetics Research: Essential for in vitro studies of DNA methylation processes and the function of methyltransferases.
• Diagnostic Assay Development: Incorporated into nucleic acid probes to improve stability, specificity, and detection in clinical diagnostic kits.
• Drug Discovery & Development: Used in the creation of aptamers and oligonucleotide-based therapeutic candidates.

Basic Information

Product Name	N(4)-Methyl-2'-Deoxycytidine 5'-Triphosphate
CAS No.	149674-24-8
Molecular Formula	C11H19N3O13P3
Molecular Weight	503.20 g/mol
Synonyms	4-Methyl-2'-deoxycytidine-5'-triphosphate; N4-Methyl-dCTP; 5'-Triphosphoric acid, 2'-deoxy-4-(methylamino)-1-(2-deoxy-β-D-erythro-pentofuranosyl)-2(1H)-pyrimidinone; 4-Methyldeoxycytidine triphosphate; N4-Methyl-2'-deoxycytidine 5'-(tetrahydrogen triphosphate); Modified dNTP; N4-Methyl-dCTP
EINECS	Contact for details

Quality Control

Our N(4)-Methyl-2'-Deoxycytidine 5'-Triphosphate is produced under stringent quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity verification, to ensure it meets the high standards required for sensitive research applications. Certificates of Analysis (COA) with detailed chromatographic data are available upon request to support your quality assurance and regulatory documentation needs.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a non-frost-free freezer. This product is hygroscopic (moisture-sensitive); allow the vial to equilibrate to room temperature before opening to prevent condensation and hydrolysis.

Specification

Item	Specification
Appearance	White to off-white solid or solution
Identification (HPLC/MS)	Conforms
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 5%
Biological Activity	Suitable for polymerase incorporation

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.