Description

Rna "G" Phosphoramidite is a high-purity, protected nucleoside phosphoramidite monomer essential for solid-phase oligonucleotide synthesis. This critical building block enables the precise and automated incorporation of guanosine (G) into RNA sequences, ensuring high coupling efficiency and fidelity. It is indispensable for researchers and manufacturers in therapeutic oligonucleotide development, diagnostic probe synthesis, and basic biochemical research where reliable and scalable RNA production is required.

Application

• Therapeutic RNA Synthesis: Production of siRNA, antisense oligonucleotides (ASOs), and other RNA-based therapeutics.
• Research Oligonucleotides: Synthesis of custom RNA primers, probes, and guides for PCR, sequencing, and CRISPR applications.
• Diagnostic Development: Manufacturing of labeled RNA probes for in situ hybridization (ISH) and microarray technologies.
• Structural Biology Studies: Preparation of specifically modified RNA strands for NMR and X-ray crystallography.
• Aptamer Discovery: Construction of RNA libraries for SELEX (Systematic Evolution of Ligands by Exponential Enrichment) processes.
• Scale-Up cGMP Manufacturing: Supports the transition from research to commercial-scale production under current Good Manufacturing Practices (cGMP).

Basic Information

Product Name	Rna "G" Phosphoramidite
CAS No.	149559-87-5
Molecular Formula	C43H54N7O8P
Molecular Weight	827.91 g/mol
Synonyms	5'-O-(4,4'-Dimethoxytrityl)-2'-O-[(tert-butyldimethylsilyl)]-N2-(Phenoxyacetyl)guanosine-3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite; Guanosine Phosphoramidite (2'-O-TBDMS protected); RNA G Phosphoramidite; DMT-G(iBu-PAC)-2'-O-TBDMS-3'-CE Phosphoramidite; 2'-O-TBDMS-rG(PAC) Phosphoramidite; Guanosine, 5'-O-[bis(4-methoxyphenyl)phenylmethyl]-2'-O-[[(1,1-dimethylethyl)dimethylsilyl]]-N2-(2-phenoxyacetyl)-, 3'-[2-cyanoethyl (diisopropylamino)phosphoramidite]
EINECS	Contact for details

Quality Control

Every batch of our Rna "G" Phosphoramidite is manufactured and tested to meet the stringent purity requirements for oligonucleotide synthesis. Our quality assurance includes rigorous analytical testing by HPLC, 31P NMR, and 1H NMR to ensure identity, purity, and stability. Certificates of Analysis (COA) with detailed chromatographic data are provided for traceability and to support your regulatory documentation needs.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere (argon or nitrogen) in a dedicated freezer. Due to its hygroscopic and strictly light-sensitive nature, the product must be handled in a dry, controlled environment and allowed to equilibrate to room temperature before opening to prevent moisture condensation.

Specification

Item	Specification
Appearance	White to off-white solid or foam
Identification (HPLC)	Conforms to reference standard
Identification (31P NMR)	Conforms to reference standard
Purity (HPLC)	≥ 98.5%
Moisture (KF Titration)	≤ 0.5%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.