Description

5'-Thiol Modifier C6 Disulfide is a high-purity, bifunctional phosphoramidite reagent designed for the automated synthesis of oligonucleotides. This compound is essential for introducing a protected thiol group at the 5'-terminus of DNA or RNA strands, enabling subsequent conjugation to surfaces, proteins, or other biomolecules. It is a critical component for researchers and manufacturers in the fields of therapeutic oligonucleotide development, diagnostic probe synthesis, and nanotechnology, where precise and stable attachment chemistry is required.

Application

• Oligonucleotide Conjugation: Primary reagent for introducing a thiol group at the 5'-end of synthetic DNA/RNA for conjugation to gold nanoparticles, maleimide-activated proteins, or other surfaces.
• Therapeutic Aptamer & siRNA Production: Used in the synthesis of oligonucleotide therapeutics that require site-specific labeling or immobilization for targeted delivery and pharmacokinetic studies.
• Diagnostic Assay Development: Key for manufacturing capture probes and signaling elements in microarray, biosensor, and lateral flow assay platforms.
• Bioconjugation & Immobilization: Enables the stable, oriented immobilization of oligonucleotides onto chips, beads, or electrodes for genomics and proteomics research.
• Materials Science: Facilitates the creation of self-assembled monolayers (SAMs) and DNA-directed assembly of nanomaterials.
• Antisense Oligonucleotide (ASO) Research: Used to attach reporter molecules or functional groups to ASOs for tracking and efficacy studies.

Basic Information

Product Name	5'-Thiol Modifier C6 Disulfide
CAS No.	148254-21-1
Molecular Formula	C41H51N6O7PS2
Molecular Weight	835.06 g/mol
Synonyms	5'-Thiol-Modifier C6 S-S; 1-[(2-Cyanoethoxy)(diisopropylamino)phosphino]-2-[[3-[(2,2,4,4-tetraisopropyldioxodiphosphoranyl)disulfanyl]propyl]carbamoyl]ethane; 5'-Thiol Modifier C6 (Disulfide); Thiol Modifier C6 S-S CPG; 5'-Disulfide Phosphoramidite; 5'-Thiolating Agent C6 Disulfide; S-Trityl-6-mercaptohexyl-1-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite (Disulfide form)
EINECS	Contact for details

Quality Control

Our 5'-Thiol Modifier C6 Disulfide is manufactured under strict quality systems to ensure batch-to-batch consistency for sensitive oligonucleotide synthesis. Each lot is subjected to rigorous analytical testing, including HPLC, 31P NMR, and 1H NMR, to confirm identity, purity, and stability. Certificates of Analysis (COA) with detailed chromatographic data are provided. Production follows GMP-like guidelines suitable for research and pre-clinical therapeutic applications.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store desiccated at -20°C or below. Under these conditions, the product is stable for at least 24 months. Allow the sealed vial to equilibrate to room temperature before opening to prevent moisture condensation.

Specification

Item	Specification
Appearance	White to off-white solid or foam
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to reference spectrum
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in anhydrous acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.