Description

Dmt-2′o-Tbdms-Rg(Ib) Phosphoramidite is a high-purity, specialty phosphoramidite monomer used in the automated solid-phase synthesis of oligonucleotides. This compound is critical for the precise and efficient introduction of modified ribonucleosides into RNA sequences, enabling advanced research and therapeutic development. It is an essential raw material for pharmaceutical companies, CROs, and academic institutions engaged in the development of siRNA, antisense oligonucleotides, and other RNA-based therapeutics and diagnostics.

Application

• Solid-Phase Oligonucleotide Synthesis: As a key building block for the automated synthesis of RNA strands containing specific modified nucleosides.
• Therapeutic RNA Production: Manufacture of siRNA (small interfering RNA) and antisense oligonucleotides (ASOs) for targeted gene silencing therapies.
• Research Grade Oligonucleotides: Synthesis of custom RNA probes, primers, and standards for molecular biology and diagnostic assay development.
• Antisense Drug Development: Incorporation into oligonucleotides designed to modulate gene expression for treating genetic disorders, cancers, and viral infections.
• Diagnostic Probe Synthesis: Production of labeled or modified RNA probes used in techniques like FISH (Fluorescence In Situ Hybridization) and microarray analysis.
• Structural Biology Studies: Creation of site-specifically modified RNA for NMR and X-ray crystallography to study RNA structure and function.

Basic Information

Product Name	Dmt-2′o-Tbdms-Rg(Ib) Phosphoramidite
CAS No.	147201-04-5
Molecular Formula	C41H53N4O8PSi
Molecular Weight	789.0 g/mol
Synonyms	5'-O-(4,4'-Dimethoxytrityl)-2'-O-(tert-butyldimethylsilyl)-N6-benzoyl-3'-O-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite-adenosine; 5'-O-DMT-2'-O-TBDMS-N6-Bz-rA Phosphoramidite; 2'-O-TBDMS-N6-Bz-5'-O-DMT-Adenosine Phosphoramidite; Adenosine, 5'-O-[bis(4-methoxyphenyl)phenylmethyl]-2'-O-[[(1,1-dimethylethyl)dimethylsilyl]-N6-benzoyl-, 3'-[(2-cyanoethyl) phosphoramidite]; DMT-2'-O-TBDMS-N6-Bz-Adenosine CE Phosphoramidite; RNA Phosphoramidite Monomer (2'-O-TBDMS, N6-Bz-A); rA(Bz) Phosphoramidite (2'-O-TBDMS)
EINECS	Contact for details

Quality Control

Our Dmt-2′o-Tbdms-Rg(Ib) Phosphoramidite is manufactured under strict quality systems to ensure batch-to-batch consistency and high purity, essential for successful oligonucleotide synthesis. Each lot undergoes comprehensive analytical testing, including HPLC for purity and identity confirmation, 31P NMR and 1H NMR for structural verification. A detailed Certificate of Analysis (COA) is provided with every shipment, documenting critical parameters such as assay purity, moisture content, and solvent residues to support your quality and regulatory requirements.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C ± 5°C under an inert atmosphere (argon or nitrogen) in a dry, freezer. This product is hygroscopic (moisture-sensitive). Allow the sealed vial to equilibrate to room temperature before opening to prevent condensation and hydrolysis.

Specification

Item	Specification
Appearance	White to off-white solid or foam
Identification (HPLC)	Conforms to reference standard
Identification (31P NMR)	Conforms to reference standard
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 0.5%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.