Description

2',3'-Dideoxy-5-Fluorocytidine 5'-Triphosphate is a modified nucleoside triphosphate analog critical for advanced biochemical research and diagnostic development. This compound is valued for its role as a chain terminator in sequencing and its potential use in studying viral polymerases. It is primarily required by research institutions, pharmaceutical R&D departments, and diagnostic kit manufacturers engaged in molecular biology, virology, and nucleotide-based therapeutics. The product is supplied to meet the stringent purity and stability demands of these sensitive applications.

Application

• DNA Sequencing & Analysis: Used as a chain-terminating substrate in Sanger sequencing and next-generation sequencing (NGS) platforms to study sequence-specific incorporation.
• Virology Research: Serves as a probe or potential inhibitor in the study of viral reverse transcriptase and DNA polymerase enzymes, particularly for viruses like HIV and Hepatitis B.
• PCR & Molecular Diagnostics: Employed in the development and optimization of specialized PCR assays and diagnostic kits that require modified nucleotides for enhanced specificity or detection.
• Biochemical Enzyme Studies: Acts as a substrate in kinetic assays to characterize the activity, fidelity, and inhibition profiles of various DNA polymerases.
• Antiviral Drug Development: Investigated as a precursor or active metabolite in the research pipeline for novel nucleoside analog antiviral agents.
• Labeling & Detection Probes: Can be utilized as a starting point for synthesizing fluorescently or radioactively labeled nucleotides for hybridization and detection applications.

Basic Information

Product Name	2',3'-Dideoxy-5-Fluorocytidine 5'-Triphosphate
CAS No.	146369-72-4
Molecular Formula	C10H15FN3O12P3
Molecular Weight	481.16 g/mol
Synonyms	5-Fluoro-ddCTP; 2',3'-Dideoxy-5-fluorocytidine triphosphate; 5-Fluoro-2',3'-dideoxycytidine 5'-triphosphate; 5-F-ddCTP; ddFCTP; 5-Fluoro-dideoxycytidine triphosphate; 1-[(2R,3S,5R)-3-Hydroxy-5-(hydroxymethyl)oxolan-2-yl]-5-fluoro-2-oxo-1,2-dihydropyrimidin-4-yl] trihydrogen triphosphate
EINECS	Contact for details

Quality Control

Our 2',3'-Dideoxy-5-Fluorocytidine 5'-Triphosphate is produced and controlled under a strict quality management system. Each batch undergoes comprehensive analytical testing, including HPLC for purity assessment, NMR and MS for structural confirmation, and tests for residual solvents and moisture content. We provide a detailed Certificate of Analysis (COA) with every shipment, ensuring traceability and compliance with your research or production specifications. Our quality commitment aligns with the standards expected for high-value biochemical reagents.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a non-frost-free freezer. Due to its hygroscopic and moisture-sensitive nature, the product should be handled in a dry environment and allowed to equilibrate to room temperature before opening to prevent condensation. For long-term stability, consider storing under an inert atmosphere to minimize oxidation.

Specification

Item	Specification
Appearance	White to off-white powder or solid
Identification (HPLC)	Retention time corresponds to reference standard
Identification (NMR)	Spectrum consistent with reference structure
Purity (HPLC)	≥ 95% (Area Percent)
Assay (UV)	90 - 110% of labeled amount
Water Content (KF)	≤ 5.0 %
Solubility	Soluble in water, slightly soluble in methanol

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.