Description

D-Luciferin 6'-o-Phosphate Trisodium Salt is a key water-soluble substrate for firefly luciferase, essential for generating bioluminescent signals. This compound is critical for high-sensitivity detection and quantification in various biochemical assays. It is primarily utilized by researchers and manufacturers in the life sciences, pharmaceutical development, and diagnostic industries for applications requiring precise, non-radioactive measurement of biological processes.

Application

• Bioluminescence Resonance Energy Transfer (BRET) Assays: Used as the light-emitting substrate to study protein-protein interactions and intracellular signaling pathways in real-time.
• ATP Detection & Quantification: Serves as the core reagent in luciferase-based kits for highly sensitive measurement of adenosine triphosphate (ATP), indicating cell viability and metabolic activity.
• Reporter Gene Assays: The standard substrate for detecting and quantifying luciferase reporter gene expression, widely used in gene regulation studies, promoter analysis, and high-throughput screening (HTS).
• In Vivo & In Vitro Imaging: Enables non-invasive bioluminescent imaging (BLI) in live animals and cell cultures for tracking tumor growth, gene expression, and therapeutic response.
• Microbial Detection: Employed in rapid hygiene monitoring and microbial contamination tests by detecting microbial ATP.
• Drug Discovery & Development: Integral to cell-based assays for screening drug candidates, assessing cytotoxicity, and studying receptor-ligand interactions.

Basic Information

Product Name	D-Luciferin 6'-o-Phosphate Trisodium Salt
CAS No.	145613-12-3
Molecular Formula	C11H7N2Na3O9P
Molecular Weight	419.11 g/mol
Synonyms	D-Luciferin 6'-Monophosphate Trisodium Salt; Firefly D-Luciferin 6'-Phosphate Trisodium Salt; D-Luciferin 6'-O-Phosphate Trisodium; D-Luciferin 6'-Phosphate Trisodium Salt; Luciferin 6'-Phosphate Trisodium Salt; D-LH2-MP Trisodium Salt; (4S)-4,5-Dihydro-2-(6-hydroxy-1,3-benzothiazol-2-yl)-4-thiazolecarboxylic Acid 6-(Dihydrogen Phosphate) Trisodium Salt
EINECS	Contact for details

Quality Control

Our D-Luciferin 6'-o-Phosphate Trisodium Salt is manufactured under strict quality systems to ensure batch-to-batch consistency and high performance in sensitive assays. Each lot undergoes comprehensive analytical testing, including HPLC for purity and identity confirmation. A Certificate of Analysis (COA) detailing purity, assay, and impurity profiles is provided with every shipment to support your quality assurance and regulatory needs.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). For long-term stability, store desiccated. Allow the vial to equilibrate to room temperature before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC/UV)	Conforms
Purity (HPLC)	≥ 95.0%
Assay (Enzymatic)	≥ 90.0%
Water Content (KF)	≤ 5.0%
Solubility	Freely soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.