Description

3'-o-(5'-Dimethoxytritylthymidyl)-5'-o-(3'-Camphanoylthymidyl)-o-Methyl Phosphite is a high-purity, specialty phosphoramidite monomer used in the automated synthesis of oligonucleotides. This compound is critical for ensuring high coupling efficiency and sequence fidelity during solid-phase DNA/RNA assembly. It is an essential raw material for research institutions, diagnostic kit manufacturers, and pharmaceutical companies developing advanced therapeutics, including antisense oligonucleotides and siRNA.

Application

• Oligonucleotide Synthesis: As a key building block (phosphoramidite) in automated solid-phase synthesizers for the production of DNA and RNA strands.
• Therapeutic Oligonucleotide Manufacturing: For the research and GMP-scale production of antisense drugs, siRNA, aptamers, and other nucleic acid-based therapeutics.
• Diagnostic Probe Development: Used in synthesizing labeled or modified probes for PCR, qPCR, FISH, and microarray technologies.
• Research & Development: Enables the creation of custom oligonucleotides with specific modifications for academic and industrial R&D in genomics and molecular biology.
• Synthesis of Modified Nucleotides: Serves as an intermediate for introducing specific protecting groups (DMT, Camphanoyl) and the methyl phosphite linkage into nucleotide analogs.

Basic Information

Product Name	3'-o-(5'-Dimethoxytritylthymidyl)-5'-o-(3'-Camphanoylthymidyl)-o-Methyl Phosphite
CAS No.	144674-88-4
Molecular Formula	C58H64N4O15P
Molecular Weight	1087.12 g/mol
Synonyms	DT-Cam-Thymidine Methyl Phosphite; 5'-O-(4,4'-Dimethoxytrityl)-2'-deoxythymidine-3'-O-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite (related precursor); Thymidine DMT-Cam Phosphoramidite Dimer; 3'-O-DMT-Thymidine-5'-O-Camphanoyl-Thymidine Methyl Phosphite; Oligonucleotide Synthesis Monomer; Nucleic Acid Phosphoramidite; CAS 144674-88-4; DMT-dT(Cam)-dT Phosphite
EINECS	Contact for details

Quality Control

This high-value synthetic intermediate is produced under strict quality control protocols suitable for pharmaceutical research. Every batch is characterized using advanced analytical techniques including HPLC, NMR, and mass spectrometry to ensure identity, purity, and reactivity. Certificates of Analysis (COA) detailing purity, moisture content, and performance in coupling tests are provided. Our manufacturing process adheres to cGMP principles where applicable, ensuring consistency and traceability for critical applications.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere (argon or nitrogen) in a desiccated environment. This compound is hygroscopic (moisture-sensitive) and easily oxidized; prolonged exposure to air, moisture, or elevated temperatures will lead to decomposition and reduced coupling efficiency. Allow the sealed vial to equilibrate to room temperature before opening to prevent condensation.

Specification

Item	Specification
Appearance	White to off-white solid or foam
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 97.0%
Water Content (KF)	≤ 0.5%
Solubility	Soluble in anhydrous acetonitrile, dichloromethane

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.