Description

5'-(Tetrahydrogen Triphosphate), 2'-o-Methyl-Cytidine is a chemically modified nucleoside triphosphate analog, specifically a cytidine derivative with a 2'-O-methyl ribose modification and a triphosphate group at the 5' position. This molecular structure is critical for its role as a building block in the synthesis of modified oligonucleotides, offering enhanced nuclease resistance and stability. It is an essential raw material for research and development in therapeutic oligonucleotide synthesis, particularly for antisense drugs, siRNA, and aptamers. Global pharmaceutical and biotechnology companies rely on this high-purity intermediate to advance next-generation genetic medicines.

Application

• Therapeutic Oligonucleotide Synthesis: Key monomer for the solid-phase synthesis of antisense oligonucleotides (ASOs) and small interfering RNA (siRNA) with improved metabolic stability.
• Research & Development: Used in biochemical research to study RNA interference (RNAi) mechanisms, enzyme kinetics, and nucleic acid-protein interactions.
• Diagnostic Probe Development: Incorporated into labeled probes for diagnostic assays, enhancing probe stability and specificity.
• Aptamer Production: Serves as a modified nucleotide in SELEX processes to generate nuclease-resistant aptamers for therapeutic and diagnostic applications.
• mRNA Vaccine & Therapy Research: Investigated for use in modified mRNA constructs to modulate immunogenicity and translation efficiency.
• Bioconjugation & Labeling: A precursor for further chemical modifications to attach fluorophores, quenchers, or other functional groups to oligonucleotides.

Basic Information

Product Name	5'-(Tetrahydrogen Triphosphate), 2'-o-Methyl-Cytidine
CAS No.	143028-98-2
Molecular Formula	C14H23N3O14P3
Molecular Weight	574.26 g/mol
Synonyms	2'-O-Methylcytidine-5'-triphosphate; 2'-OMe-CTP; N4-Benzoyl-2'-O-methyl-5'-O-triphosphocytidine; 2'-O-Methyl-CTP; Cytidine, 2'-O-methyl-, 5'-(tetrahydrogen triphosphate); 5'-Triphosphoric acid, 2'-O-methylcytidine; 2'-Methylcytidine triphosphate
EINECS	Contact for details

Quality Control

Our 5'-(Tetrahydrogen Triphosphate), 2'-o-Methyl-Cytidine is manufactured under strict quality systems suitable for pharmaceutical research applications. Each batch undergoes rigorous analytical testing to ensure identity, purity, and consistency. We provide comprehensive Certificates of Analysis (COA) with detailed HPLC, NMR, and MS data. Our quality commitment aligns with the standards required for GMP-grade starting materials in oligonucleotide therapeutics.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This material is hygroscopic (moisture-sensitive). For long-term storage, desiccate under inert atmosphere. Allow the sealed container to reach room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	White to off-white powder or solid
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 5.0%
Triphosphate Content	≥ 85%
Related Substances (HPLC)	Individual impurity ≤ 2.0%
Biological Activity	Suitable for enzymatic incorporation

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.