Description

n-Benzoyl-2-Deoxy-5-o-Dmt-2',2'-Difluorocytidine 3-Ce Phosphoramidite CAS NO 142808-44-4 is a high-purity, protected phosphoramidite monomer specifically engineered for oligonucleotide synthesis. This compound is a critical building block for the automated solid-phase synthesis of DNA and RNA sequences, particularly those requiring modified nucleosides. It is essential for researchers and manufacturers in the fields of therapeutic oligonucleotides, antisense drugs, siRNA, and molecular diagnostics, where precise and reliable synthesis is paramount.

Application

• Solid-Phase Oligonucleotide Synthesis: As a key phosphoramidite monomer for the automated synthesis of DNA and RNA strands.
• Therapeutic Oligonucleotide Manufacturing: Production of antisense oligonucleotides (ASOs), small interfering RNA (siRNA), and aptamers for pharmaceutical applications.
• Research & Development: Synthesis of custom oligonucleotide probes, primers, and gene fragments for molecular biology and genetic research.
• Diagnostic Reagent Production: Manufacturing of labeled probes and primers used in PCR, qPCR, and next-generation sequencing (NGS) diagnostic kits.
• Synthesis of Modified Nucleosides: Incorporation of 2',2'-difluorocytidine analogs into oligonucleotide chains to alter stability, binding affinity, or pharmacokinetic properties.

Basic Information

Product Name	n-Benzoyl-2-Deoxy-5-o-Dmt-2',2'-Difluorocytidine 3-Ce Phosphoramidite
CAS No.	142808-44-4
Molecular Formula	C46H49F2N4O8P
Molecular Weight	870.88 g/mol
Synonyms	5'-O-DMT-2'-Deoxy-2',2'-difluorocytidine, 3'-[(2-Cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite, N4-Benzoyl-5'-O-(4,4'-dimethoxytrityl)-2'-deoxy-2',2'-difluorocytidine-3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite; 2'-Deoxy-2',2'-difluorocytidine Phosphoramidite; dFdC Phosphoramidite; Gemcitabine Intermediate Phosphoramidite; 5'-O-Dimethoxytrityl-N4-benzoyl-2'-deoxy-2',2'-difluorocytidine, 3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite
EINECS	Contact for details

Quality Control

Our n-Benzoyl-2-Deoxy-5-o-Dmt-2',2'-Difluorocytidine 3-Ce Phosphoramidite is manufactured under strict quality systems to ensure batch-to-batch consistency and high purity suitable for sensitive oligonucleotide synthesis. Each lot undergoes comprehensive analytical testing, including HPLC, NMR, and 31P NMR analysis, to confirm identity, purity, and reactivity. Certificates of Analysis (COA) with detailed chromatographic data are provided to support your GMP or research-grade applications.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere (argon or nitrogen) in a desiccated environment. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the sealed vial to equilibrate to room temperature before opening to prevent condensation.

Specification

Item	Specification
Appearance	White to off-white solid or foam
Identification (HPLC)	Conforms to reference standard
Identification (31P NMR)	Conforms to reference standard
Purity (HPLC)	≥ 97.0%
Moisture (KF)	≤ 1.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.