Description

3'-Biotinyl-5'-Fluorosceinyldodecakis(α-Deoxythymidylate) is a sophisticated, dual-labeled oligonucleotide probe designed for high-sensitivity detection and analysis. This compound matters because it combines the high-affinity binding of biotin with the intense fluorescence of fluorescein, enabling versatile applications in molecular diagnostics and life science research. It is primarily needed by biotechnology companies, diagnostic kit manufacturers, and academic research institutions engaged in advanced nucleic acid hybridization, fluorescence in situ hybridization (FISH), and microarray technologies. The product is supplied to exacting standards to ensure reliable performance in critical assays.

Application

• Fluorescence In Situ Hybridization (FISH): Used as a high-sensitivity probe for the detection and localization of specific DNA sequences on chromosomes or within cells.
• Microarray and Biochip Technology: Serves as a critical component in the fabrication of DNA microarrays for genomic and diagnostic screening.
• Molecular Diagnostics: Employed in the development of diagnostic assays for genetic disorders, pathogens, and cancer biomarkers.
• Nucleic Acid Detection & Purification: The biotin moiety allows for efficient capture and purification via streptavidin-biotin interaction, while fluorescein enables visualization.
• Advanced Research Probes: Utilized in cutting-edge research applications such as single-molecule detection and super-resolution microscopy.
• Quality Control for Oligonucleotide Synthesis: Acts as a reference standard or control probe in the quality assessment of synthetic oligonucleotide batches.

Basic Information

Product Name	3'-Biotinyl-5'-Fluorosceinyldodecakis(α-Deoxythymidylate)
CAS No.	141436-87-5
Molecular Formula	C148H190N26O78P12S
Molecular Weight	~3980.0 g/mol (Contact for exact value)
Synonyms	Biotin-Fluorescein-dT12; Biotinylated Fluorescein-labeled dodecathymidylate; 5'-Fluorescein, 3'-Biotin labeled (dT)12; Fluorescein-Biotin-(dT)12 conjugate; Dual-labeled dT12 oligonucleotide; F-dT12-Biotin; Biotin-3'-(dT)12-5'-Fluorescein; Oligonucleotide Probe (Biotin, Fluorescein)
EINECS	Contact for details

Quality Control

Our 3'-Biotinyl-5'-Fluorosceinyldodecakis(α-Deoxythymidylate) is manufactured under stringent controls to ensure batch-to-batch consistency and high purity, essential for reproducible research and diagnostic results. Each lot undergoes comprehensive analytical testing, including HPLC for purity assessment, mass spectrometry for identity confirmation, and absorbance measurements to verify labeling efficiency. Certificates of Analysis (COA) detailing all specifications are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a non-frost-free freezer. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature before opening to minimize moisture uptake. For long-term storage, consider storing under an inert atmosphere.

Specification

Item	Specification
Appearance	Yellow to orange lyophilized solid or solution
Identification (HPLC/MS)	Conforms to reference standard
Purity (HPLC)	≥ 95%
Assay (UV-Vis)	90% - 110% of stated concentration
Fluorescein Labeling Efficiency	≥ 0.9 mol Fluorescein / mol Oligo
Biotin Labeling Efficiency	≥ 0.9 mol Biotin / mol Oligo
Oligonucleotide Length	12-mer (dT)
Solubility	Soluble in water, TE buffer, or DMSO

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.