Description

(N4-Benzoyl)-5'-O-[(N,N-Diisopropylamino)-(2-Cyanoethoxy)Phosphinyl]-3'-O-(4,4'-Dimethoxytrityl)-2'-Deoxycytidine is a highly specialized, phosphoramidite-protected nucleoside monomer, a critical building block in automated solid-phase oligonucleotide synthesis. Its value lies in enabling the precise, high-yield incorporation of a protected deoxycytidine unit into custom DNA sequences. This compound is essential for researchers and manufacturers in the fields of therapeutic oligonucleotide development, diagnostic probe synthesis, and genetic research.

Application

• Solid-Phase Oligonucleotide Synthesis: As a key phosphoramidite monomer for the automated synthesis of DNA strands in antisense, siRNA, and aptamer development.
• Therapeutic Oligonucleotide Manufacturing: Production of active pharmaceutical ingredients (APIs) for gene silencing, exon skipping, and other nucleic acid-based therapies.
• Diagnostic Probe and Primer Synthesis: Fabrication of labeled and unlabeled DNA probes for PCR, sequencing, microarray, and in-situ hybridization applications.
• Research-Grade DNA Synthesis: Enabling academic and industrial research labs to construct custom gene fragments, primers, and molecular tools.
• Synthesis of Modified Oligonucleotides: Foundation for creating oligonucleotides with site-specific modifications for enhanced stability, binding affinity, or detection.

Basic Information

Product Name	(N4-Benzoyl)-5'-O-[(N,N-Diisopropylamino)-(2-Cyanoethoxy)Phosphinyl]-3'-O-(4,4'-Dimethoxytrityl)-2'-Deoxycytidine
CAS No.	140712-83-0
Molecular Formula	C53H58N5O9P
Molecular Weight	940.03 g/mol
Synonyms	5'-O-(Bis(N,N-diisopropylamino)(2-cyanoethoxy)phosphino)-2'-deoxy-N4-benzoyl-3'-O-(4,4'-dimethoxytrityl)cytidine; dC(Bz) Phosphoramidite (DMTr protected); N4-Benzoyl-2'-deoxycytidine-3'-O-(4,4'-dimethoxytrityl)-5'-O-[(2-cyanoethyl)-(N,N-diisopropyl)]phosphoramidite; dC(Bz) CE Phosphoramidite; 2'-Deoxycytidine, N4-benzoyl-3'-O-[bis(4-methoxyphenyl)phenylmethyl]-5'-O-[[[2-cyanoethoxy(diisopropylamino)phosphino]oxy]-; dCbz Phosphoramidite; Cytidine Phosphoramidite (Bz, DMTr)
EINECS	Contact for details

Quality Control

Our nucleoside phosphoramidites are manufactured under strict quality systems to ensure the high purity and consistency required for critical oligonucleotide synthesis. Each batch undergoes comprehensive analytical testing, including HPLC for purity and 31P NMR for structural confirmation, to meet stringent in-house specifications. A detailed Certificate of Analysis (COA) is provided with every shipment, documenting key parameters such as assay, moisture content, and solvent residues.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere (argon or nitrogen) in a desiccated environment. This product is hygroscopic (moisture-sensitive) and easily oxidized; prolonged exposure to air, moisture, or elevated temperatures will lead to degradation and reduced coupling efficiency.

Specification

Item	Specification
Appearance	White to off-white solid or foam
Identification (HPLC)	Conforms to reference standard
Identification (31P NMR)	Conforms to reference standard
Assay (HPLC)	≥ 97.0%
Water Content (KF)	≤ 0.5%
Solvent Residues (GC)	Meet ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.