Description

(N6-Benzoyl)-5'-O-[(N,N-Diisopropylamino)-(2-Cyanoethoxy)Phosphinyl]-3'-O-(4,4'-Dimethoxytrityl)-2'-Deoxyadenosine is a highly specialized phosphoramidite monomer, a critical building block for the automated solid-phase synthesis of oligonucleotides. Its value lies in providing precise control over the incorporation of a protected deoxyadenosine nucleotide, enabling the production of high-purity DNA sequences for research and diagnostics. This compound is essential for biotechnology, pharmaceutical, and academic laboratories engaged in the development of primers, probes, antisense oligonucleotides, and other synthetic nucleic acid applications.

Application

• Oligonucleotide Synthesis: As a key phosphoramidite monomer for the automated synthesis of DNA strands in solid-phase synthesizers.
• Research & Development: For creating custom DNA primers, probes, and gene fragments used in PCR, sequencing, and molecular biology studies.
• Therapeutic Nucleic Acid Development: In the synthesis of antisense oligonucleotides, siRNA, and aptamers for pharmaceutical research.
• Diagnostic Reagent Production: Manufacturing labeled probes and components for diagnostic kits and microarray technologies.
• Synthetic Biology: Building DNA constructs and genetic circuits for engineered biological systems.
• High-Throughput Genomics: Supporting large-scale DNA synthesis for next-generation sequencing and functional genomics platforms.

Basic Information

Product Name	(N6-Benzoyl)-5'-O-[(N,N-Diisopropylamino)-(2-Cyanoethoxy)Phosphinyl]-3'-O-(4,4'-Dimethoxytrityl)-2'-Deoxyadenosine
CAS No.	140712-82-9
Molecular Formula	C53H58N7O8P
Molecular Weight	951.04 g/mol
Synonyms	5'-O-(4,4'-Dimethoxytrityl)-N6-benzoyl-2'-deoxyadenosine-3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite; dA(Bz) Phosphoramidite, DMT-protected; N6-Benzoyl-2'-deoxyadenosine-3'-(2-cyanoethyl diisopropylphosphoramidite), 5'-DMT; dABz CE Phosphoramidite; 2'-Deoxyadenosine, N-benzoyl-5'-O-[bis(4-methoxyphenyl)phenylmethyl]-3'-O-[2-[[bis(1-methylethyl)amino](2-cyanoethoxy)phosphinyl]ethyl]-; Adenosine phosphoramidite, N-benzoyl-2'-deoxy-5'-O-(4,4'-dimethoxytrityl)-3'-O-[2-[[bis(1-methylethyl)amino](2-cyanoethoxy)phosphino]ethyl]-
EINECS	Contact for details

Quality Control

Our phosphoramidite monomers are produced under strict quality-controlled environments to ensure the high purity and consistency required for sensitive oligonucleotide synthesis. Each batch is characterized by advanced analytical techniques including HPLC, 31P NMR, and 1H NMR to confirm identity, purity, and the absence of critical impurities. A comprehensive Certificate of Analysis (COA) detailing assay, moisture content, and solvent residues is provided with every shipment to support your quality assurance protocols.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere (argon or nitrogen) in a desiccated environment. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the sealed vial to equilibrate to room temperature before opening to prevent moisture condensation.

Specification

Item	Specification
Appearance	White to off-white solid or foam
Identification (HPLC)	Conforms to reference standard
Identification (31P NMR)	Conforms to reference standard
Assay (HPLC)	≥ 98.0%
Water Content (KF)	≤ 0.5%
Solvent Residues (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.