Description

N4-Benzoyl-3'-o-(4,4'-Dimethoxytrityl)-2'-Deoxycytidine is a critical, high-purity protected nucleoside phosphoramidite building block used in the automated synthesis of oligonucleotides. This compound is essential for ensuring high coupling efficiency and sequence fidelity during solid-phase DNA/RNA synthesis. It is primarily required by manufacturers and research institutions in the pharmaceutical, biotechnology, and diagnostic sectors for applications in antisense therapy, PCR primers, probes, and next-generation sequencing.

Application

• Oligonucleotide Synthesis: As a key phosphoramidite monomer for the automated solid-phase synthesis of DNA strands.
• Therapeutic Oligonucleotide Production: Manufacturing of antisense oligonucleotides (ASOs), siRNA, and aptamers for drug development.
• Diagnostic Probe Fabrication: Synthesis of labeled probes for PCR, qPCR, FISH, and microarray technologies.
• Next-Generation Sequencing (NGS): Production of primers and adapters used in library preparation for genomic sequencing.
• Research & Development: Academic and industrial R&D for studying gene function, expression, and regulation.
• Synthetic Biology: Construction of synthetic genes and genetic circuits.

Basic Information

Product Name	N4-Benzoyl-3'-o-(4,4'-Dimethoxytrityl)-2'-Deoxycytidine
CAS No.	140712-80-7
Molecular Formula	C43H39N3O7
Molecular Weight	709.79 g/mol
Synonyms	5'-O-(4,4'-Dimethoxytrityl)-N4-benzoyl-2'-deoxycytidine; dC(Bz) DMT; N4-Benzoyl-5'-O-(4,4'-dimethoxytrityl)-2'-deoxycytidine; 2'-Deoxycytidine, N4-benzoyl-5'-O-[bis(4-methoxyphenyl)phenylmethyl]-; DMT-dC(Bz) Amidite precursor; N-Bz-5'-DMT-2'-deoxycytidine; Protected Deoxycytidine Monomer; dCBz DMT
EINECS	Contact for details

Quality Control

Our N4-Benzoyl-3'-o-(4,4'-Dimethoxytrityl)-2'-Deoxycytidine is manufactured under strict quality systems to meet the exacting standards of oligonucleotide synthesis. Each batch is subjected to rigorous analytical testing, including HPLC for purity and identity confirmation by NMR and MS. We provide comprehensive Certificates of Analysis (COA) with each shipment, detailing purity, moisture content, and solvent residues to ensure batch-to-batch consistency and compliance with your internal specifications.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C or as specified on the label or COA. This material is hygroscopic (moisture-sensitive); ensure containers are sealed under an inert atmosphere (argon or nitrogen) after opening to maintain stability and prevent degradation.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 0.5%
Solvent Residues (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.