Description

(2R,3S,5R)-5-(4-Amino-2-Oxopyrimidin-1(2H)-Yl)-2-((Bis(4-Methoxyphenyl)(Phenyl)Methoxy)Methyl)Tetrahydrofuran-3-Yl 2-Cyanoethyl Diisopropylphosphoramidite is a high-purity phosphoramidite monomer, a critical building block in automated oligonucleotide synthesis. This compound is essential for the precise and efficient assembly of DNA and RNA sequences, enabling the production of primers, probes, and therapeutic oligonucleotides. It is primarily utilized by research institutions, diagnostic developers, and pharmaceutical companies engaged in molecular biology, genomics, and antisense drug development.

Application

• Solid-Phase Oligonucleotide Synthesis: As a key phosphoramidite reagent for the automated synthesis of DNA and RNA strands.
• Therapeutic Oligonucleotide Manufacturing: Production of antisense oligonucleotides (ASOs), siRNA, and aptamers for pharmaceutical applications.
• Molecular Diagnostics: Synthesis of specific probes and primers for PCR, qPCR, and next-generation sequencing (NGS) applications.
• Genomics Research: Creation of custom oligonucleotides for gene editing (CRISPR), cloning, and mutagenesis studies.
• Bioconjugation and Labeling: Serving as an intermediate for the synthesis of modified nucleotides with fluorescent or affinity tags.
• Antisense Technology Development: Research and development of novel nucleic acid-based therapeutics and tools.

Basic Information

Product Name	(2R,3S,5R)-5-(4-Amino-2-Oxopyrimidin-1(2H)-Yl)-2-((Bis(4-Methoxyphenyl)(Phenyl)Methoxy)Methyl)Tetrahydrofuran-3-Yl 2-Cyanoethyl Diisopropylphosphoramidite
CAS No.	140613-57-6
Molecular Formula	C43H49N4O7P
Molecular Weight	748.85 g/mol
Synonyms	5'-O-DMT-2'-deoxyCytidine-3'-CE Phosphoramidite; dC-CE Phosphoramidite; 2'-Deoxycytidine-5'-O-(4,4'-dimethoxytrityl)-N4-benzoyl-3'-O-(2-cyanoethyl-N,N-diisopropyl)phosphoramidite (common protected form); dC(Bz) Phosphoramidite; Deoxycytidine Phosphoramidite; DNA Synthesis Monomer (dC); Cytidine Phosphoramidite Building Block
EINECS	Contact for details

Quality Control

Our phosphoramidite monomers are manufactured under strict quality control protocols to ensure the high purity and consistency required for sensitive oligonucleotide synthesis. Each batch is analyzed via HPLC, 31P NMR, and 1H NMR to confirm identity, purity, and the absence of critical impurities. Certificates of Analysis (COA) detailing all specifications are provided with every shipment. Production can adhere to GMP guidelines for therapeutic-grade material upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere (argon or nitrogen) in a dry, moisture-free environment. This product is hygroscopic (moisture-sensitive) and easily oxidized; prolonged exposure to air or ambient temperature will lead to degradation. Allow the sealed container to equilibrate to room temperature before opening to prevent condensation.

Specification

Item	Specification
Appearance	White to off-white solid or foam
Identification (HPLC)	Conforms to reference standard
Identification (31P NMR)	Conforms to reference standard
Purity (HPLC)	≥ 98.5%
Water Content (KF)	≤ 0.5%
Solubility	Clear, colorless solution in anhydrous acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.