Description

Biotin-16-dUTP *1 mM in Tris Buffer (pH 7.5)* is a nucleotide analog specifically designed for the enzymatic incorporation of biotin labels into nucleic acids. This product is essential for researchers requiring high-sensitivity detection and capture in molecular biology applications. It is primarily used by professionals in life sciences, diagnostics, and biotechnology for techniques such as in situ hybridization, microarray fabrication, and next-generation sequencing library preparation. The ready-to-use formulation in a stabilized buffer ensures consistent performance and convenience.

Application

• Non-Radiometric Labeling of DNA/RNA Probes: For safe and stable detection in Southern, Northern, and dot/slot blot hybridizations.
• In Situ Hybridization (ISH) and Fluorescence In Situ Hybridization (FISH): Enables precise localization of nucleic acid sequences within cells and tissues.
• Microarray and Biochip Fabrication: Used to create high-density arrays for gene expression profiling and genomic analysis.
• Next-Generation Sequencing (NGS): Facilitates the preparation of biotinylated adapters or libraries for target enrichment and pull-down assays.
• DNA/Protein Interaction Studies: Useful for creating labeled DNA fragments in electrophoretic mobility shift assays (EMSAs) or chromatin pull-down experiments.
• Diagnostic Assay Development: A key component in the development of sensitive molecular diagnostic kits and biosensors.

Basic Information

Product Name	Biotin-16-dUTP *1 mM in Tris Buffer (pH 7.5)*
CAS No.	136632-31-0
Molecular Formula	C₃₅H₅₀N₆O₁₉P₃
Molecular Weight	990.73 g/mol
Synonyms	Biotin-16-2'-deoxyuridine-5'-triphosphate; Biotin-16-2'-dUTP; 5-(Biotin-16-amidocaproylamidopropyl)-2'-deoxyuridine 5'-triphosphate; Biotinylated dUTP; Biotin-16-AA-dUTP; 2'-Deoxyuridine-5'-triphosphate, 5-[N-[N-(biotinyl)-6-aminocaproyl]-3-aminopropyl]-, trisodium salt
EINECS	Contact for details

Quality Control

Our Biotin-16-dUTP is manufactured under stringent quality systems to ensure batch-to-batch consistency and reliability for critical research. Each lot is tested to confirm identity, purity, and functional performance. A comprehensive Certificate of Analysis (COA) detailing specifications such as concentration, buffer composition, and absence of contaminating nucleases is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at -20°C or below. Avoid repeated freeze-thaw cycles. The product is supplied in a light-sensitive (store away from light) format.

Specification

Item	Specification
Appearance	Clear, colorless to slightly yellow solution
Concentration	1 mM ± 10%
Buffer	Tris-HCl, pH 7.5 ± 0.2
Identification (HPLC)	Conforms to reference standard
Purity (HPLC)	≥ 95%
Functional Test	Suitable for enzymatic incorporation
Endotoxin	< 0.1 EU/µmol (if applicable)
Nuclease Activity	Not detected

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.