Description

Phenol:Chloroform:Isoamyl Alcohol 25:24:1 is a specialized molecular biology reagent, a precisely formulated mixture for the efficient extraction and purification of nucleic acids. Its core value lies in its ability to effectively separate DNA and RNA from proteins and other cellular contaminants in a single step. This product is essential for research and diagnostic laboratories, biotechnology firms, and pharmaceutical manufacturers requiring high-purity genetic material for downstream applications like PCR, sequencing, and cloning.

Application

• Nucleic Acid Extraction: Primary reagent for the isolation of high-quality DNA, RNA, and plasmid DNA from complex biological samples such as tissues, cells, and blood.
• Molecular Biology Research: Critical for protocols in genomics, transcriptomics, and cloning where pure nucleic acid templates are required.
• Diagnostic Test Development: Used in the preparation of samples for PCR-based diagnostic assays, including pathogen detection and genetic testing.
• Biopharmaceutical Production: Employed in quality control and process development for the purification of nucleic acid-based therapeutics and vaccines.
• Forensic Analysis: Aids in the extraction of DNA from challenging forensic samples for genetic fingerprinting.
• Plant and Agricultural Genomics: Effective for isolating nucleic acids from plant tissues, which often contain high levels of polysaccharides and polyphenols.

Basic Information

Product Name	Phenol:Chloroform:Isoamyl Alcohol 25:24:1
CAS No.	136112-00-0
Molecular Formula	Mixture (C6H6O / CHCl3 / C5H12O)
Molecular Weight	Mixture
Synonyms	Phenol-Chloroform-IAA; PCI; PCI Reagent; Phenol/Chloroform/Isoamyl Alcohol (25:24:1); DNA/RNA Extraction Reagent; Acid Phenol:Chloroform:IAA; Phenol:Chloroform:3-Methyl-1-butanol; Nucleic Acid Extraction Mixture; Trizol-like Reagent (component)
EINECS	Contact for details

Quality Control

Our Phenol:Chloroform:Isoamyl Alcohol 25:24:1 is manufactured under strict quality systems to ensure batch-to-batch consistency and performance for sensitive molecular biology applications. Each lot is tested to confirm the precise ratio of components, purity, and the absence of contaminants like nucleases that could degrade nucleic acids. A comprehensive Certificate of Analysis (COA) detailing specifications and test results is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, well-ventilated area at 2-8°C (refrigerated). Due to its highly volatile and flammable nature, keep away from heat, sparks, and open flames. Containers must be upright and securely sealed to prevent leakage and evaporation.

Specification

Item	Specification
Appearance	Clear, colorless to pale yellow liquid
Identification (IR)	Conforms to standard for Phenol, Chloroform & Isoamyl Alcohol
Composition Ratio (Phenol:Chloroform:IAA)	25 : 24 : 1 (v/v)
Phenol Content (Assay)	≥ 99.0%
Chloroform Content (Assay)	≥ 99.0%
Isoamyl Alcohol Content (Assay)	≥ 98.0%
Water Content	≤ 0.5%
pH (Aqueous Layer)	4.0 - 5.0
Nuclease Activity	Not detected
Functionality Test (Nucleic Acid Recovery)	≥ 95% recovery of control DNA/RNA

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.