Description

Tris Edta Acetate Buffer, 10X, Dnase, Rnase And Protease Free, Ph 8.3, For Molecular Biology is a high-purity, concentrated buffer solution specifically formulated for critical nucleic acid electrophoresis and blotting applications. Its core value lies in providing a nuclease and protease-free environment, which is essential for preventing the degradation of sensitive DNA and RNA samples during analysis. This product is indispensable for researchers and technicians in molecular biology, genomics, and diagnostic laboratories who require reliable, contaminant-free buffers for gel electrophoresis, Southern blotting, and Northern blotting to ensure the integrity of their experimental results.

Application

• Agarose Gel Electrophoresis of Nucleic Acids: Primary buffer for running DNA and RNA fragments in agarose gels, providing optimal separation and resolution.
• Southern Blotting: Used as the running and transfer buffer for the capillary transfer of DNA from gels to membrane supports.
• Northern Blotting: Employed in the electrophoresis and blotting procedures for RNA analysis.
• Molecular Cloning Workflows: A key reagent for analyzing restriction enzyme digests and PCR products prior to purification or cloning.
• Quality Control in Biomanufacturing: Used to verify the size and integrity of plasmid DNA, oligonucleotides, and other nucleic acid-based products.
• Diagnostic Assay Development: Provides a stable, contaminant-free buffer matrix for developing and running electrophoresis-based clinical diagnostics.

Basic Information

Item	Detail
Product Name	Tris Edta Acetate Buffer, 10X, Dnase, Rnase And Protease Free, Ph 8.3, For Molecular Biology
CAS No.	135852-26-5
Molecular Formula	Mixture (C4H11NO3, C10H16N2O8, C2H4O2)
Molecular Weight	N/A (Buffer Solution)
Synonyms	10X TAE Buffer; Tris-Acetate-EDTA Buffer, 10X Concentrate; TAE Running Buffer, 10X; Electrophoresis Buffer, 10X TAE; Tris Acetate EDTA Buffer, Molecular Biology Grade; DNA Electrophoresis Buffer; RNase-free TAE Buffer; DNase-free Tris Acetate EDTA
EINECS	Contact for details

Quality Control

This molecular biology grade buffer is manufactured under strict quality control protocols to ensure it meets the stringent requirements of life science research. Every batch is tested to be free of detectable DNase, RNase, and protease activities. Certificates of Analysis (COA) are provided, detailing specifications for pH, conductivity, absorbance, and functional performance in electrophoresis. Our quality system is designed to support compliance with relevant ISO standards.

Storage

Preserve in a tightly closed container, protected from light. Store at room temperature (15-25°C). For long-term stability, avoid repeated warming and cooling cycles. The solution is stable under these conditions for the period stated on the label.

Specification

Item	Specification
Appearance	Clear, colorless solution
Concentration	10X Concentrate
pH (at 25°C, 1X)	8.3 ± 0.2
DNase Activity	Not detected
RNase Activity	Not detected
Protease Activity	Not detected
Conductivity	Conforms
Absorbance (at 260nm, 1X)	< 0.05
Functional Test (Electrophoresis)	Conforms

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.