Description

3'-Mercapto-3'-Deoxythymidine-5'-Triphosphate is a modified nucleotide triphosphate where the 3'-hydroxyl group of the deoxyribose sugar is replaced by a thiol (-SH) functionality. This compound is critical for research and development in molecular biology, particularly as a substrate for DNA polymerases in specialized applications like chain termination sequencing and site-directed mutagenesis. It is primarily utilized by biotechnology firms, academic research institutions, and pharmaceutical companies engaged in nucleic acid chemistry and next-generation sequencing workflows.

Application

• Next-Generation Sequencing (NGS): Used as a reversible terminator in sequencing-by-synthesis platforms to enable base-specific incorporation and detection.
• Site-Directed Mutagenesis: Acts as a substrate for DNA polymerases to introduce specific point mutations during in vitro DNA synthesis.
• DNA Labeling and Detection: The thiol group provides a handle for conjugation with fluorescent dyes or other reporter molecules for probe development.
• Enzymatic Synthesis of Modified Oligonucleotides: Essential for the enzymatic production of oligonucleotides containing thiol-modified nucleotides for structural studies or therapeutic aptamers.
• Biochemical Research: Serves as a tool to study DNA polymerase kinetics, fidelity, and mechanism when incorporating modified nucleotides.
• Diagnostic Assay Development: Incorporated into nucleic acid probes for enhanced stability or novel detection mechanisms in clinical diagnostics.

Basic Information

Product Name	3'-Mercapto-3'-Deoxythymidine-5'-Triphosphate
CAS No.	135490-62-9
Molecular Formula	C10H17N2O13P3S
Molecular Weight	498.23 g/mol
Synonyms	3'-Thio-dTTP; 3'-S-dTTP; 2'-Deoxy-3'-thioribothymidine-5'-triphosphate; 3'-Deoxy-3'-thioribothymidine-5'-triphosphate; 3'-Thio-2',3'-dideoxythymidine-5'-triphosphate; dTTP-3'-S; 3'-Mercapto-ddTTP
EINECS	Contact for details

Quality Control

Our 3'-Mercapto-3'-Deoxythymidine-5'-Triphosphate is manufactured under strict quality systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity and identity verification and NMR for structural confirmation, to ensure it meets the stringent requirements for molecular biology applications. Certificates of Analysis (COA) with detailed chromatographic data are provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere (e.g., argon or nitrogen) to prevent oxidation and hydrolysis. The material is hygroscopic (moisture-sensitive); allow the vial to equilibrate to room temperature before opening to minimize moisture ingress.

Specification

Item	Specification
Appearance	White to off-white solid or lyophilized powder
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 3.0%
Triphosphate Content	≥ 85% (by HPLC or P-NMR)
Biological Activity	Suitable for enzymatic incorporation (tested with selected polymerase)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.