Description

Fluorescein-12-UTP is a fluorescently labeled uridine triphosphate nucleotide analog, a critical reagent for advanced molecular biology and diagnostic applications. Its core value lies in enabling the sensitive, direct detection and quantification of RNA synthesis and related enzymatic activities without the need for radioactive isotopes. This product is essential for researchers and manufacturers in molecular diagnostics, next-generation sequencing (NGS) probe synthesis, and fluorescence in situ hybridization (FISH).

Application

• Fluorescent Labeling of RNA Transcripts: Direct incorporation into RNA by RNA polymerases (e.g., T7, SP6) for non-radioactive detection in transcription assays and microarray analysis.
• Next-Generation Sequencing (NGS): Used in the synthesis of fluorescently labeled probes and primers for target enrichment and library preparation.
• Fluorescence In Situ Hybridization (FISH): Synthesis of high-sensitivity FISH probes for the visualization and spatial mapping of gene expression in cells and tissues.
• Enzyme Activity Assays: Real-time, continuous monitoring of RNA polymerase, poly(A) polymerase, and other nucleotide-utilizing enzyme activities in high-throughput screening (HTS).
• Single-Molecule Detection: Critical for single-molecule fluorescence experiments, including single-molecule RNA imaging and super-resolution microscopy techniques.
• Diagnostic Kit Manufacturing: A key raw material for producing in vitro diagnostic (IVD) kits for viral detection, cancer biomarkers, and genetic disorders.

Basic Information

Product Name	Fluorescein-12-UTP
CAS No.	134367-01-4
Molecular Formula	C35H35FN3O22P3
Molecular Weight	961.57 g/mol
Synonyms	5-(3-Aminoallyl)-UTP-fluorescein; 5-Aminoallyl-UTP, Fluorescein labeled; AA-UTP-Fluorescein; Fluorescein-12-2'-deoxyuridine-5'-triphosphate; 5-(3-Aminoallyl)-2'-deoxyuridine-5'-triphosphate, fluorescein conjugate; Fluorescein-12-dUTP (common misnomer); FluoroLink Cy3-UTP (related trade name category); UTP-ε-AA-FITC
EINECS	Contact for details

Quality Control

Our Fluorescein-12-UTP is manufactured under strict quality systems to ensure batch-to-batch consistency and performance in sensitive applications. Each lot is characterized by HPLC for purity and functionality. Certificates of Analysis (COA) are provided, detailing specifications for identity, purity (HPLC), fluorescence properties, and absence of contaminating nucleases and phosphatases. We support compliance with ISO 13485 standards for diagnostic manufacturers.

Storage

Preserve in a tightly closed container, protected from light. Store desiccated at -20°C or below. This product is hygroscopic (moisture-sensitive) and light-sensitive. For long-term storage, keep under an inert atmosphere. Allow the vial to equilibrate to room temperature before opening to prevent condensation.

Specification

Item	Specification
Appearance	Yellow to orange solid or solution
Identification (HPLC/UV-Vis)	Conforms to reference standard
Purity (HPLC)	≥ 90% (Fluorescein-labeled UTP)
Free Fluorescein	≤ 2.0%
Free Nucleotides	≤ 5.0%
Extinction Coefficient (ε)	~78,000 cm⁻¹M⁻¹ at 494 nm (in buffer)
Emission Maximum	~518 nm (Excitation: 494 nm)
Solubility	Soluble in aqueous buffer (pH 7.5), DMSO
Enzymatic Incorporation	Suitable for RNA polymerase reactions

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.