Description

Fluorescein-11-Deoxyuridine Triphosphate is a fluorescently labeled nucleotide analog designed for sensitive detection and analysis in molecular biology. This compound is critical for applications requiring high-resolution visualization of nucleic acid synthesis and enzymatic activity. It is primarily utilized by researchers and diagnostic developers in life sciences, genomics, and pharmaceutical development for advanced labeling and probing techniques.

Application

• DNA Sequencing & Labeling: Used as a fluorescently labeled dUTP for next-generation sequencing (NGS) platforms and Sanger sequencing, enabling real-time detection.
• Fluorescence In Situ Hybridization (FISH): Serves as a direct label for probe synthesis in cytogenetic and pathological diagnostics.
• Polymerase Chain Reaction (PCR): Incorporated into amplicons during PCR for highly sensitive, fluorescence-based detection and quantification.
• Enzyme Activity Assays: Acts as a substrate for DNA polymerases, reverse transcriptases, and terminal transferases in kinetic and mechanistic studies.
• Microarray Technology: Employed in the synthesis of fluorescently labeled cDNA or cRNA targets for gene expression profiling.
• Single-Molecule Detection: Essential for super-resolution microscopy and single-molecule tracking experiments in biophysical research.
• Apoptosis Detection (TUNEL Assay): Used to label nicked DNA in cells for the detection of programmed cell death.
• Probe Development: A key reagent for creating sensitive and specific nucleic acid probes for diagnostic and research applications.

Basic Information

Product Name	Fluorescein-11-Deoxyuridine Triphosphate
CAS No.	134344-32-4
Molecular Formula	C31H33N4O19P3
Molecular Weight	882.53 g/mol
Synonyms	Fluorescein-11-dUTP; 5-(3-Aminoallyl)-2'-deoxyuridine-5'-triphosphate, fluorescein conjugate; Fluorescein-11-2'-deoxyuridine-5'-triphosphate; 2'-Deoxy-5-[(3-{[(5-fluoresceinyl)amino]carbonyl}propyl)amino]uridine 5'-(trihydrogen triphosphate); Alexa Fluor 488-dUTP (similar class); Fluorescent dUTP; F-11-dUTP; Fluorescein-labeled dUTP
EINECS	Contact for details

Quality Control

Our Fluorescein-11-Deoxyuridine Triphosphate is manufactured under strict quality systems to ensure batch-to-batch consistency and performance. Each lot undergoes comprehensive analytical testing, including HPLC for purity, spectrophotometry for labeling efficiency and concentration, and functional testing in enzymatic incorporation assays. Certificates of Analysis (COA) with detailed specifications are provided to support your research and regulatory requirements.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a non-frost-free freezer. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature before opening to minimize moisture uptake. For long-term storage, consider under an inert atmosphere.

Specification

Item	Specification
Appearance	Orange to red solid or solution
Identification (HPLC/MS)	Conforms to structure
Purity (HPLC)	≥ 95%
Absorption Maximum (λmax)	~495 nm
Emission Maximum	~518 nm
Extinction Coefficient (ε)	~68,000 cm⁻¹M⁻¹
Solubility	Soluble in water, TE buffer (pH 7.5-8.0)
Enzymatic Incorporation	Suitable for use with DNA polymerases

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.