Description

5'-o-[(N,n-Diisopropylamino)-(2-Cyanoethoxy)Phosphinyl]-3'-o-(4,4'-Dimethoxytrityl)-Thymidine is a highly specialized phosphoramidite monomer, a critical building block in automated solid-phase oligonucleotide synthesis. Its primary value lies in enabling the precise, sequential addition of thymidine nucleotides to create custom DNA strands. This compound is essential for research institutions, diagnostic developers, and pharmaceutical companies engaged in the production of oligonucleotides for PCR primers, probes, gene synthesis, and therapeutic applications.

Application

• Oligonucleotide Synthesis: As a thymidine phosphoramidite for the automated synthesis of DNA strands.
• Therapeutic Oligonucleotide Manufacturing: Production of antisense oligonucleotides, siRNA, and aptamers for drug development.
• Molecular Diagnostics: Synthesis of primers and probes for PCR, qPCR, and next-generation sequencing applications.
• Genetic Research: Creation of custom gene fragments, mutagenesis primers, and synthetic genes.
• Biotechnology Reagent Production: Manufacture of labeled nucleotides and other research-grade biochemicals.

Basic Information

Item	Detail
Product Name	5'-o-[(N,n-Diisopropylamino)-(2-Cyanoethoxy)Phosphinyl]-3'-o-(4,4'-Dimethoxytrityl)-Thymidine
CAS No.	134031-86-0
Molecular Formula	C41H49N4O8P
Molecular Weight	756.83 g/mol
Synonyms	dT Phosphoramidite (DMT); 5'-Dimethoxytrityl-thymidine-3'-[2-cyanoethyl-(N,N-diisopropyl)]-phosphoramidite; Thymidine 3'-(2-cyanoethyl N,N-diisopropylphosphoramidite) 5'-(4,4'-dimethoxytrityl) ether; DMT-dT-CE Phosphoramidite; T Phosphoramidite; Amidite T; dT-CE Phosphoramidite; 5'-DMT-Thymidine-3'-phosphoramidite
EINECS	Contact for details

Quality Control

Our 5'-o-[(N,n-Diisopropylamino)-(2-Cyanoethoxy)Phosphinyl]-3'-o-(4,4'-Dimethoxytrityl)-Thymidine is manufactured under strict quality systems suitable for pharmaceutical and molecular biology applications. Each batch undergoes rigorous analytical testing, including HPLC purity analysis and 31P NMR to confirm identity and reactivity. Certificates of Analysis (COA) with detailed specifications are provided to ensure traceability and batch-to-batch consistency for your critical synthesis workflows.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C under an inert atmosphere (argon or nitrogen) in a dry, moisture-free environment. This compound is hygroscopic (moisture-sensitive) and easily oxidized; prolonged exposure to air or moisture will degrade product quality and performance. Allow the sealed vial to equilibrate to room temperature before opening to prevent condensation.

Specification

Item	Specification
Appearance	White to off-white powder or foam
Identification (HPLC)	Conforms to reference standard
Identification (31P NMR)	Conforms to reference standard
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 0.5%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.