Description

8-Allyloxy-3'-O-[(Diisopropylamino)(2-Cyanoethoxy)Phosphino]-5'-O-(4,4'-Dimethoxytrityl)-N2-[(Dimethylamino)Methylidine]-2'-Deoxyguanosine is a highly specialized, phosphoramidite-protected nucleoside derivative, a critical building block in automated oligonucleotide synthesis. Its value lies in enabling the precise, large-scale production of modified DNA and RNA sequences for advanced research and therapeutic applications. This compound is essential for biotechnology, pharmaceutical R&D, and diagnostic developers requiring high-purity, sequence-specific nucleic acid probes, primers, and therapeutic oligonucleotides.

Application

• Solid-Phase Oligonucleotide Synthesis: As a key phosphoramidite monomer for the automated synthesis of modified DNA strands.
• Therapeutic Oligonucleotide Production: Manufacturing of antisense oligonucleotides, siRNA, and aptamers for drug development.
• Diagnostic Probe Fabrication: Synthesis of labeled probes for genetic testing, fluorescence in situ hybridization (FISH), and microarray technologies.
• Primer Synthesis for PCR: Creation of specialized primers with modified bases for enhanced specificity or detection in polymerase chain reaction (PCR) applications.
• Basic Biochemical Research: Tool for studying nucleic acid structure, function, and protein-nucleic acid interactions.
• Synthesis of Antisense Agents: Direct production of research-grade antisense molecules for gene function studies.

Basic Information

Product Name	8-Allyloxy-3'-O-[(Diisopropylamino)(2-Cyanoethoxy)Phosphino]-5'-O-(4,4'-Dimethoxytrityl)-N2-[(Dimethylamino)Methylidine]-2'-Deoxyguanosine
CAS No.	133783-15-0
Molecular Formula	C48H61N8O8P
Molecular Weight	909.03 g/mol
Synonyms	8-Allyloxy-dG-CE Phosphoramidite; 5'-O-(4,4'-Dimethoxytrityl)-N2-(dimethylaminomethylene)-8-allyloxy-2'-deoxyguanosine-3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite; dG(8-allyl)-CE Phosphoramidite; N2-Dimethylaminomethylene-8-allyloxy-2'-deoxyguanosine-3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite; 8-Allyl-2'-deoxyguanosine Phosphoramidite; Guanosine, 2'-deoxy-, N2-[(dimethylamino)methylene]-8-(2-propen-1-yloxy)-, 5'-[bis(4-methoxyphenyl)phenylmethyl] 3'-[2-cyanoethyl bis(1-methylethyl)phosphoramidite]
EINECS	Contact for details

Quality Control

Our 8-Allyloxy-dG-CE Phosphoramidite is manufactured under strict quality-controlled conditions suitable for sensitive oligonucleotide synthesis. Each batch undergoes comprehensive analytical testing, including HPLC, 1H/31P NMR, and mass spectrometry, to ensure high purity and consistent performance. A detailed Certificate of Analysis (COA) is provided with every shipment, confirming compliance with our stringent in-house specifications for critical parameters like moisture content and phosphoramidite purity.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere (argon or nitrogen) in a dry, moisture-free environment. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the sealed vial to equilibrate to room temperature before opening to prevent condensation.

Specification

Item	Specification
Appearance	White to off-white solid or foam
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 97.0%
Water Content (KF)	≤ 0.5%
31P NMR Purity	≥ 98.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.