Description

5'-O-(4,4'-Dimethoxytrityl)-O6-Phenyl-2'-Deoxyinosine, 3'-[(2-Cyanoethyl)-(N,N-Diisopropyl)]Phosphoramidite is a high-purity, specialty phosphoramidite monomer essential for the solid-phase synthesis of oligonucleotides. This compound is critical for introducing modified nucleosides into DNA sequences, enabling advanced research and therapeutic development. It is primarily used by biotechnology and pharmaceutical companies engaged in antisense therapy, diagnostic probe development, and fundamental nucleic acid research.

Application

• Solid-Phase Oligonucleotide Synthesis: Key building block for automated DNA/RNA synthesizers.
• Antisense Oligonucleotide (ASO) Production: Synthesis of therapeutic oligonucleotides targeting specific mRNA sequences.
• Diagnostic Probe Manufacture: Creation of labeled probes for PCR, FISH, and microarray technologies.
• Research & Development: Tool for studying nucleic acid structure, function, and protein interactions.
• Synthesis of Modified DNA: Incorporation of 2'-deoxyinosine analogs to introduce degeneracy or specific binding properties.
• Next-Generation Sequencing (NGS): Preparation of specialized oligonucleotide libraries and adapters.

Basic Information

Product Name	5'-O-(4,4'-Dimethoxytrityl)-O6-Phenyl-2'-Deoxyinosine, 3'-[(2-Cyanoethyl)-(N,N-Diisopropyl)]Phosphoramidite
CAS No.	133471-07-5
Molecular Formula	C46H51N6O7P
Molecular Weight	830.92 g/mol
Synonyms	5'-O-DMT-O6-Phenyl-2'-deoxyinosine 3'-CE phosphoramidite; DMT-dI(Ph) Phosphoramidite; 2'-Deoxyinosine, 5'-O-[bis(4-methoxyphenyl)phenylmethyl]-O6-phenyl-, 3'-[2-cyanoethyl bis(1-methylethyl)phosphoramidite]; O6-Phenyl-2'-deoxyinosine phosphoramidite; dI(Ph) Phosphoramidite; 5'-O-(4,4'-Dimethoxytrityl)-O6-phenyl-deoxyinosine-3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite
EINECS	Contact for details

Quality Control

This high-value phosphoramidite is manufactured under strict quality control protocols to ensure batch-to-batch consistency and optimal coupling efficiency. Every lot is characterized by HPLC, ¹H/³¹P NMR, and mass spectrometry to confirm identity and purity. Certificates of Analysis (COA) detailing purity, moisture content, and functional performance are provided with each shipment. Our production adheres to cGMP principles where applicable for research-grade materials.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere (argon or nitrogen) in a desiccated environment. This compound is hygroscopic (moisture-sensitive) and easily oxidized. Allow the sealed vial to equilibrate to room temperature before opening to prevent condensation and hydrolysis.

Specification

Item	Specification
Appearance	White to off-white solid or foam
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 97.0%
Moisture Content (KF)	≤ 1.0%
Solubility	Soluble in anhydrous acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.