Description

5-Chloro-2'-Deoxyuridine 5'-Triphosphate (CAS NO 132183-42-7) is a halogenated deoxyribonucleoside triphosphate (dNTP) analog, a critical building block for advanced molecular biology and diagnostic applications. This modified nucleotide is engineered to introduce specific functionalities into nucleic acid chains, enabling precise control in enzymatic synthesis and analysis. It is an essential reagent for researchers and manufacturers in molecular diagnostics, next-generation sequencing (NGS), polymerase chain reaction (PCR) applications, and antiviral drug development.

Application

• Molecular Diagnostics: Used as a substrate in PCR and other amplification techniques for the detection of specific genetic sequences and mutations.
• Next-Generation Sequencing (NGS): Serves as a chain-terminating or modified nucleotide for sequencing-by-synthesis platforms to study genomics and epigenetics.
• Antiviral Research: Investigated as a potential prodrug or active metabolite in the development of nucleoside analog therapies targeting viral polymerases.
• Enzymatic DNA Synthesis: Incorporated by DNA polymerases for the in vitro synthesis of oligonucleotides with specific chemical modifications.
• Biochemical Studies: Employed as a tool to probe enzyme mechanisms, study DNA repair pathways, and investigate polymerase fidelity.
• Labeling & Detection: Can be used to introduce tags or labels into DNA for sensitive detection and imaging applications.

Basic Information

Product Name	5-Chloro-2'-Deoxyuridine 5'-Triphosphate
CAS No.	132183-42-7
Molecular Formula	C10H15ClN2O13P3
Molecular Weight	523.61 g/mol
Synonyms	5-Chloro-dUTP; 5-Cl-dUTP; 2'-Deoxy-5-chlorouridine 5'-triphosphate; 5-Chloro-2'-deoxyuridine triphosphate; Cl-dUTP; 5-Chloro-2'-deoxyuridine-5'-triphosphate; 5-Chloro-2'-deoxyuridine 5'-(tetrahydrogen triphosphate)
EINECS	Contact for details

Quality Control

Our 5-Chloro-2'-Deoxyuridine 5'-Triphosphate is manufactured under stringent quality systems to ensure the high purity and consistency required for sensitive life science applications. Each batch is subjected to rigorous analytical testing, including HPLC for purity assessment and identity confirmation. A comprehensive Certificate of Analysis (COA) detailing purity, impurity profile, and concentration is provided with every shipment to support your quality assurance and regulatory compliance needs.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a non-frost-free freezer. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature before opening to minimize moisture absorption. For long-term storage, consider under an inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white solid or solution
Identification (HPLC/UV)	Conforms
Purity (HPLC)	≥ 95%
Assay (Enzymatic)	≥ 90%
Water Content (KF)	≤ 3.0%
pH (for solutions)	6.5 - 7.5

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.