Description

n-Blocked-5'-o-Dmt-2'-o-Fpmp Ced Guanosine Phosphoramidite is a high-purity, chemically modified nucleoside phosphoramidite building block essential for solid-phase oligonucleotide synthesis. This compound is critically important for the automated production of research-grade and therapeutic oligonucleotides, where precise control over sequence and modification is paramount. It is primarily needed by biotechnology and pharmaceutical companies engaged in the development of antisense oligonucleotides, siRNAs, aptamers, and other advanced nucleic acid-based therapeutics and diagnostics.

Application

• Solid-Phase Oligonucleotide Synthesis: As a key monomer for the automated, stepwise assembly of custom DNA/RNA sequences on solid support.
• Therapeutic Oligonucleotide Manufacturing: Production of active pharmaceutical ingredients (APIs) for antisense, siRNA, and miRNA-based drugs.
• Research-Grade Probe Synthesis: Creation of labeled or modified oligonucleotide probes for genomics, diagnostics, and basic research applications.
• Aptamer Development: Synthesis of chemically modified nucleic acid libraries for SELEX processes to identify high-affinity binding molecules.
• Synthesis of Modified RNAs: Specifically for incorporating 2'-O-Fpmp protected guanosine residues, which offer orthogonal protection strategies for complex RNA synthesis.
• Scale-Up cGMP Production: Serves as a critical raw material in the cGMP-compliant manufacturing of oligonucleotides for clinical trials and commercial supply.

Basic Information

Product Name	n-Blocked-5'-o-Dmt-2'-o-Fpmp Ced Guanosine Phosphoramidite
CAS No.	131474-76-5
Molecular Formula	C₄₇H₅₃FN₇O₈P
Molecular Weight	893.94 g/mol
Synonyms	5'-O-(4,4'-Dimethoxytrityl)-2'-O-[(R)-1-(2-Fluorophenyl)-4-methoxypiperidin-4-yl]-N2-(dimethylaminomethylene)guanosine-3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite; 2'-O-Fpmp-G(Ibu) Phosphoramidite; Guanosine, 2'-O-[(1R)-1-(2-fluorophenyl)-4-methoxypiperidin-4-yl]-5'-O-[bis(4-methoxyphenyl)phenylmethyl]-N2-[(dimethylamino)methylene]-, 3'-[2-cyanoethyl bis(1-methylethyl)phosphoramidite]; Ced Phosphoramidite of 5'-DMT-2'-Fpmp-Guanosine; N-Blocked DMT-Fpmp-G Phosphoramidite
EINECS	Contact for details

Quality Control

Our n-Blocked-5'-o-Dmt-2'-o-Fpmp Ced Guanosine Phosphoramidite is manufactured under strict quality systems suitable for demanding research and pre-clinical applications. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation (typically ≥98%), to ensure consistency and reliability. Certificates of Analysis (COA) with full chromatographic data are provided with every shipment. We support customers requiring materials for cGMP manufacturing with appropriate documentation and quality agreements.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere (argon or nitrogen) in a desiccated environment. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the sealed vial to equilibrate to room temperature before opening to prevent condensation and moisture ingress.

Specification

Item	Specification
Appearance	White to off-white solid or foam
Identification (HPLC)	Retention time corresponds to reference standard
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 0.5%
Solubility	Clear, colorless solution in anhydrous acetonitrile
31P NMR	Consistent with phosphoramidite structure

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.