Description

n-(5-Maleimido-3-Oxapentyl)(2-(3-Carboxybenzoyl)Thio)Acetylglycyl-Glycyl-Glycinamide is a specialized heterobifunctional crosslinking agent designed for precise biomolecular conjugation. This compound is engineered to facilitate stable, site-specific coupling between proteins, peptides, or other biomolecules, making it a critical tool in advanced research and biopharmaceutical development. It is essential for scientists and manufacturers in the fields of bioconjugation, antibody-drug conjugate (ADC) development, and diagnostic assay fabrication who require reliable and efficient crosslinking chemistry.

Application

• Antibody-Drug Conjugate (ADC) Linker: A key component for constructing stable, cleavable linkers between cytotoxic payloads and monoclonal antibodies.
• Protein-Protein/Peptide Conjugation: Enables the site-specific crosslinking of different biomolecules for creating novel protein complexes or multi-functional constructs.
• Surface Immobilization: Used to functionalize sensor chips, nanoparticles, or chromatographic resins with biomolecules for biosensing, diagnostics, or affinity purification.
• Fluorescent Probe & Diagnostic Reagent Synthesis: Serves as a linker to attach reporter molecules (e.g., fluorophores, enzymes) to targeting agents like antibodies or peptides.
• Polymer Modification: Facilitates the grafting of bioactive peptides or proteins onto polymer backbones to create smart biomaterials.
• Research Tool in Chemical Biology: Employed in studying protein interactions, mapping binding sites, and developing novel therapeutic modalities.

Basic Information

Product Name	n-(5-Maleimido-3-Oxapentyl)(2-(3-Carboxybenzoyl)Thio)Acetylglycyl-Glycyl-Glycinamide
CAS No.	131274-05-0
Molecular Formula	C25H29N5O11S
Molecular Weight	607.59 g/mol
Synonyms	Maleimide-PEG2-SS-COOH; 3-((2-(2-(3-(2,5-Dioxo-2,5-dihydro-1H-pyrrol-1-yl)propoxy)ethoxy)ethyl)disulfanyl)-3-oxopropyl)benzoic acid-Gly-Gly-Gly-NH2; SM(PEG)2 crosslinker derivative; Heterobifunctional PEG crosslinker, Maleimide and Carboxyl; N-[5-Maleimido-3-oxapentyl]-S-[2-(3-carboxybenzoyl)thio]acetylglycylglycylglycinamide; Acetyl-Gly-Gly-Gly-NH- linker with maleimide and cleavable disulfide; (3-Carboxybenzoic acid)-SS-Maleimide crosslinker.
EINECS	Contact for details

Quality Control

Our n-(5-Maleimido-3-Oxapentyl)(2-(3-Carboxybenzoyl)Thio)Acetylglycyl-Glycyl-Glycinamide is manufactured under strict quality systems to ensure batch-to-batch consistency and high purity, critical for sensitive bioconjugation applications. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and functional performance. We support development under cGMP guidelines where required and ensure compliance with relevant global regulatory frameworks for research and pharmaceutical use.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This compound is hygroscopic (moisture-sensitive) and the maleimide group is susceptible to hydrolysis. For long-term storage, keep under an inert atmosphere. Allow the sealed vial to equilibrate to room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC/MS)	Conforms to structure
Identification (IR)	Conforms to reference
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF; Slightly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.