5-Bromo-4-Chloro-3-Indolyl Butyrate CAS NO 129541-43-1

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CAS No.:129541-43-1

Grade:Pharmacy Grade

Content:99.9%

Brand:Customizable

Packaging:Customizable

Description

5-Bromo-4-Chloro-3-Indolyl Butyrate is a chromogenic substrate widely used in enzymatic assays and histochemical staining. This compound is valued for its role in producing an insoluble blue precipitate upon enzymatic cleavage, providing a clear visual or spectrophotometric signal. It is primarily needed by researchers and manufacturers in the life sciences, diagnostic, and biotechnology industries for applications in molecular biology and diagnostic test development.

Application

As a key substrate for the detection of β-galactosidase activity in molecular cloning (e.g., blue-white screening).
In histochemistry and cytochemistry for localizing enzyme activity in tissue sections and cell cultures.
For the development of diagnostic kits and assays requiring a sensitive colorimetric readout.
In microbiology for the identification of bacterial species based on specific enzymatic profiles.
As a reagent in plant biology research to study gene expression and promoter activity.
For quality control in the production of enzymes and other biochemical reagents.

Basic Information


Product Name	5-Bromo-4-Chloro-3-Indolyl Butyrate
CAS No.	129541-43-1
Molecular Formula	C12H11BrClNO2
Molecular Weight	316.57 g/mol
Synonyms	X-Gal Butyrate; 5-Bromo-4-chloro-3-indolyl butanoate; BCIP Butyrate; 4-Chloro-5-bromo-3-indolyl butyrate; Indoxyl butyrate, 5-bromo-4-chloro-; 3-Indolyl butyrate, 5-bromo-4-chloro-; Butanoic acid, 5-bromo-4-chloro-3-indolyl ester
EINECS	Contact for details

Quality Control

Our 5-Bromo-4-Chloro-3-Indolyl Butyrate is manufactured under strict quality systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation, to ensure it meets the high standards required for research and diagnostic applications. A comprehensive Certificate of Analysis (COA) detailing purity, assay, and impurity profiles is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 15-25°C. The product is light-sensitive and should be handled accordingly to maintain stability.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Melting Point	Contact for details
Loss on Drying	≤ 1.0%
Heavy Metals	≤ 20 ppm
Solubility	Soluble in DMF, DMSO; slightly soluble in ethanol