Description

3'-Tbdms-Bz-Ra Phosphoramidite is a specialized, high-purity building block used in the automated synthesis of oligonucleotides. This compound matters because it enables the precise and efficient incorporation of modified nucleosides, which are critical for developing advanced therapeutic and diagnostic agents. It is primarily needed by biotechnology and pharmaceutical companies engaged in the research and production of antisense oligonucleotides, siRNA, aptamers, and other nucleic acid-based technologies. Its consistent quality is paramount for ensuring the success and reproducibility of complex synthetic sequences.

Application

• Solid-Phase Oligonucleotide Synthesis: As a key phosphoramidite monomer for the automated synthesis of DNA/RNA strands.
• Therapeutic Oligonucleotide Development: For the production of antisense drugs, siRNA, and other nucleic acid therapeutics requiring specific modifications.
• Diagnostic Probe Manufacturing: Used to create labeled probes for genetic testing, fluorescence in situ hybridization (FISH), and microarray applications.
• Aptamer Research: Enables the synthesis of chemically modified aptamers with enhanced stability and binding affinity.
• Primer and Guide RNA Synthesis: For creating specialized primers and synthetic guide RNAs (sgRNAs) used in CRISPR-Cas9 gene editing workflows.
• Basic Biochemical Research: A critical reagent in academic and industrial labs studying nucleic acid structure, function, and interactions.

Basic Information

Item	Detail
Product Name	3'-Tbdms-Bz-Ra Phosphoramidite
CAS No.	129451-75-8
Molecular Formula	C41H51N6O7PSi
Molecular Weight	799.02 g/mol
Synonyms	1-[2-Cyanoethoxy(diisopropylamino)phosphino]-2'-deoxy-3'-O-[(1,1-dimethylethyl)dimethylsilyl]-N6-benzoyladenosine; 3'-O-TBDMS-N6-Bz-2'-deoxyadenosine Phosphoramidite; dA(Bz)-3'-TBDMS Phosphoramidite; 3'-O-(tert-Butyldimethylsilyl)-N6-benzoyl-2'-deoxyadenosine 2-Cyanoethyl N,N-Diisopropylphosphoramidite; N6-Benzoyl-3'-O-TBDMS-2'-deoxyadenosine Phosphoramidite; 3'-TBDMS-N6-Bz-dA Phosphoramidite; Amidite dA(Bz) 3'-TBDMS
EINECS	Contact for details

Quality Control

Our 3'-Tbdms-Bz-Ra Phosphoramidite is manufactured under strict quality systems to ensure it meets the exacting standards required for oligonucleotide synthesis. Every batch undergoes rigorous analytical testing, including HPLC for purity and NMR for structural confirmation. Certificates of Analysis (COA) with detailed specifications are provided for each lot to guarantee traceability, consistency, and performance in your critical applications.

Storage

Preserve in a tightly closed container, protected from light. Store in a freezer at -20°C under an inert atmosphere (argon or nitrogen) due to its hygroscopic and moisture-sensitive nature. Allow the sealed vial to equilibrate to room temperature before opening to prevent condensation and moisture ingress.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98.5%
Water Content (KF)	≤ 0.5%
Solubility	Clear, colorless solution in anhydrous acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.