Description

5-(3-(5-(2,2,6,6-Tetramethyl-1-Oxypiperidine-4-Carboxamido)Pentanamido)Prop-1-Enyl)-2'-Deoxycytidine 5'-Triphosphate is a specialized, chemically modified nucleotide triphosphate analog designed for advanced molecular biology and diagnostic applications. This compound is critical for applications requiring high-fidelity labeling and detection, offering enhanced stability and specificity. It is primarily utilized by research institutions, pharmaceutical developers, and diagnostic kit manufacturers working in next-generation sequencing (NGS), polymerase chain reaction (PCR), and nucleic acid labeling.

Application

• Next-Generation Sequencing (NGS): Used as a modified dCTP substrate for the incorporation of stable, detectable labels during sequencing-by-synthesis workflows.
• Polymerase Chain Reaction (PCR): Employed in the synthesis of labeled DNA probes for real-time PCR (qPCR) and other amplification-based detection assays.
• Nucleic Acid Labeling & Detection: Serves as a key reagent for the enzymatic incorporation of haptens, fluorophores, or other reporter groups into DNA.
• Diagnostic Assay Development: A vital component in the manufacture of in vitro diagnostic (IVD) kits for genetic testing and pathogen detection.
• Bioconjugation & Probe Synthesis: The reactive prop-enyl and TEMPO-based side chain facilitate further chemical modifications and cross-linking strategies.
• Biochemical Research: Used in studies of DNA polymerase kinetics, fidelity, and in the development of novel enzymatic assays.

Basic Information

Product Name	5-(3-(5-(2,2,6,6-Tetramethyl-1-Oxypiperidine-4-Carboxamido)Pentanamido)Prop-1-Enyl)-2'-Deoxycytidine 5'-Triphosphate
CAS No.	129215-94-7
Molecular Formula	C₃₀H₄₆N₇O₁₇P₃
Molecular Weight	893.66 g/mol
Synonyms	5-(3-(5-(2,2,6,6-Tetramethyl-1-oxylpiperidine-4-carboxamido)pentanamido)prop-1-en-1-yl)-2'-deoxycytidine 5'-triphosphate; TEMPO-labeled dCTP; Spin-labeled dCTP; Nitroxide-labeled deoxycytidine triphosphate; 5-(3-(5-(2,2,6,6-Tetramethylpiperidin-1-oxyl-4-carboxamido)pentanamido)allyl)-dCTP; Modified nucleotide triphosphate; Allylamine dCTP derivative; EPR-active dNTP
EINECS	Contact for details

Quality Control

Every batch of our 5-(3-(5-(2,2,6,6-Tetramethyl-1-Oxypiperidine-4-Carboxamido)Pentanamido)Prop-1-Enyl)-2'-Deoxycytidine 5'-Triphosphate is manufactured and tested under strict quality systems. We guarantee high purity and batch-to-batch consistency through comprehensive analytical protocols including HPLC, NMR, and MS. Certificates of Analysis (COA) detailing purity, identity, and performance in enzymatic assays are provided with each shipment to ensure compliance with your research and production standards.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below. This product is hygroscopic (moisture-sensitive). For long-term storage, it is recommended to store desiccated under an inert atmosphere. Allow the vial to equilibrate to room temperature before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white solid or lyophilized powder
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 3.0%
Enzymatic Incorporation	Suitable for use (tested with DNA polymerase)
Solubility	Soluble in aqueous buffer, DMSO

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.