Description

3'-o-T-Butyldimethylsilyl-5'-o-(4,4'-Dimethoxytrityl)-N4-Acetyl Cytidine is a key protected nucleoside derivative, specifically designed for the controlled synthesis of oligonucleotides. This compound is critical for ensuring high coupling efficiency and regioselectivity during automated solid-phase synthesis, directly impacting the yield and purity of the final nucleic acid product. It is an essential building block for research and development laboratories, pharmaceutical companies, and CROs engaged in the production of antisense oligonucleotides, siRNA, aptamers, and diagnostic probes.

Application

• Oligonucleotide Synthesis: Serves as a protected cytidine phosphoramidite precursor for automated solid-phase DNA/RNA synthesis.
• Antisense Therapeutics: A critical raw material in the manufacturing of antisense oligonucleotides for targeted gene silencing therapies.
• siRNA Production: Used in the synthesis of small interfering RNA (siRNA) sequences for research and therapeutic applications.
• Diagnostic Probe Development: Enables the creation of labeled and modified nucleic acid probes for molecular diagnostics and bioassays.
• Aptamer Research: Facilitates the synthesis of modified DNA/RNA libraries for the selection of high-affinity aptamers.
• Chemical Biology Studies: Employed in the site-specific incorporation of modified cytidines to study nucleic acid structure and function.

Basic Information

Product Name	3'-o-T-Butyldimethylsilyl-5'-o-(4,4'-Dimethoxytrityl)-N4-Acetyl Cytidine
CAS No.	123956-65-0
Molecular Formula	C38H47N3O8Si
Molecular Weight	701.89 g/mol
Synonyms	5'-O-(4,4'-Dimethoxytrityl)-N4-acetyl-3'-O-(tert-butyldimethylsilyl)cytidine; N4-Acetyl-5'-O-(4,4'-dimethoxytrityl)-3'-O-(tert-butyldimethylsilyl)cytidine; 3'-O-TBDMS-5'-O-DMT-N4-Acetyl Cytidine; DMT-N4-Ac-Cytidine-3'-O-TBDMS; AcN-Cytidine(DMT-TBDMS); Protected Cytidine Monomer; Cytidine Phosphoramidite Intermediate
EINECS	Contact for details

Quality Control

Our 3'-o-T-Butyldimethylsilyl-5'-o-(4,4'-Dimethoxytrityl)-N4-Acetyl Cytidine is manufactured under strict quality systems to ensure batch-to-batch consistency for sensitive oligonucleotide synthesis. Each lot is subjected to rigorous analytical testing, including HPLC, NMR, and mass spectrometry, to confirm identity, purity, and the absence of critical impurities like free nucleobases or protecting group failures. Certificates of Analysis (COA) with full chromatographic data are provided to support your quality assurance protocols.

Storage

Preserve in a tightly closed container, protected from light. Store in a freezer at -20°C or below under anhydrous conditions. This material is hygroscopic (moisture-sensitive). Allow the sealed container to equilibrate to room temperature before opening to prevent condensation. For long-term storage, consider storage under an inert atmosphere such as argon or nitrogen.

Specification

Item	Specification
Appearance	White to off-white powder or solid
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Residual Solvents (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.