Description

Restriction Endonuclease Nsi I is a high-purity, site-specific endonuclease enzyme critical for molecular biology research and biotechnological applications. Its primary value lies in its ability to recognize and cleave DNA at the specific palindromic sequence 5'-ATGCAT-3', generating cohesive ends essential for precise genetic manipulation. This product is indispensable for researchers and professionals in molecular cloning, genetic engineering, recombinant DNA technology, and genomic analysis, ensuring reliable and reproducible results in complex experimental workflows.

Application

• Molecular Cloning: Essential for the precise digestion of DNA inserts and vectors to create compatible ends for ligation.
• Restriction Fragment Length Polymorphism (RFLP) Analysis: Used for DNA fingerprinting, genotyping, and mutation detection.
• Recombinant DNA Construction: A key tool in genetic engineering for assembling novel DNA constructs and plasmids.
• Genomic DNA Mapping: Facilitates the physical mapping of genomes by generating specific DNA fragments.
• Diagnostic Assay Development: Employed in the design and validation of molecular diagnostic tests for pathogens or genetic disorders.
• Gene Synthesis and Assembly: Used in modern synthetic biology workflows for the modular assembly of genetic parts.
• Quality Control in Biomanufacturing: Verifies plasmid identity and integrity in upstream bioprocessing.

Basic Information

Product Name	Restriction Endonuclease Nsi I
CAS No.	122097-02-3
Molecular Formula	Protein (Enzyme)
Molecular Weight	Contact for details
Synonyms	NsiI; Endodeoxyribonuclease NsiI; NsiI Restriction Enzyme; Restriction Enzyme NsiI; Type II Site-Specific Deoxyribonuclease NsiI; EC 3.1.21.4
EINECS	Contact for details

Quality Control

Our Restriction Endonuclease Nsi I is manufactured under stringent quality systems to ensure exceptional activity, specificity, and purity, free from contaminating nucleases and phosphatases. Each lot is rigorously tested for specific activity, star activity, and fidelity under recommended buffer conditions. A comprehensive Certificate of Analysis (COA) detailing purity (via SDS-PAGE), specific activity units, and storage buffer composition is provided with every shipment to guarantee batch-to-batch consistency for your critical research.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at -20°C ± 5°C in a non-frost-free freezer. The enzyme is supplied in a stabilized storage buffer (typically containing 50% glycerol) to prevent freezing at -20°C. Avoid repeated freeze-thaw cycles; for frequent use, store an aliquot at 2-8°C for up to one month.

Specification

Item	Specification
Appearance	Clear, colorless solution
Identification (Activity)	Cleaves λ DNA at the specific sequence 5'-ATGCAT-3'
Specific Activity	≥ 10,000 units/mg protein
Purity (SDS-PAGE)	≥ 95% single band
Contaminating Activities	Free of detectable non-specific endonuclease, exonuclease, and phosphatase activities
Storage Buffer	10 mM Tris-HCl, 50 mM KCl, 1 mM DTT, 0.1 mM EDTA, 50% Glycerol, pH 7.4 at 25°C
Unit Definition	One unit is the amount of enzyme required to completely digest 1 µg of λ DNA in 1 hour at 37°C in a total reaction volume of 50 µl.

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.