Description

N(7)-Ethyldeoxyguanosine 5'-Triphosphate is a chemically modified nucleotide triphosphate analog, specifically an alkylated derivative of deoxyguanosine triphosphate (dGTP). This compound is of significant value in biochemical and molecular biology research as a tool for studying DNA damage, repair mechanisms, and the structural and functional consequences of specific base modifications. It is primarily utilized by research institutions, pharmaceutical R&D departments, and diagnostic developers focused on mutagenesis, carcinogenesis, and the development of novel analytical assays.

Application

• DNA Damage & Repair Studies: Used as a substrate to investigate the enzymatic processing of alkylated DNA lesions by repair polymerases and glycosylases.
• Mutagenesis Research: Employed in in vitro replication assays to study the mutagenic potential and replication fidelity past specific DNA adducts.
• Biochemical Probe Development: Serves as a critical reagent in the development of sensitive detection methods for DNA alkylation damage.
• Enzyme Kinetics & Specificity: Acts as a modified nucleotide substrate to characterize the substrate specificity and inhibition of DNA polymerases and other nucleic acid-processing enzymes.
• Structural Biology: Used in crystallography and NMR studies to understand the structural impact of N7-alkylguanine lesions on DNA conformation and protein-DNA interactions.
• Standards & Calibrants: Functions as a high-purity analytical standard for the quantification of etheno-DNA adducts in biological samples via LC-MS/MS or other chromatographic techniques.

Basic Information

Product Name	N(7)-Ethyldeoxyguanosine 5'-Triphosphate
CAS No.	122055-03-2
Molecular Formula	C₁₇H₂₇N₅O₁₃P₃
Molecular Weight	626.36 g/mol
Synonyms	7-Ethyl-2'-deoxyguanosine-5'-triphosphate; N7-Ethyldeoxyguanosine triphosphate; N7-Ethyl-dGTP; 7-Ethyl-dGTP; 2'-Deoxy-N7-ethylguanosine-5'-triphosphate; dGTP-N7-Et; N7-Et-dGTP; Alkylated dGTP
EINECS	Contact for details

Quality Control

Our N(7)-Ethyldeoxyguanosine 5'-Triphosphate is manufactured under stringent conditions to ensure the highest standards of purity and batch-to-batch consistency, suitable for sensitive research applications. Each batch is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and stability data. We adhere to rigorous internal quality protocols, and specifications can be aligned with customer-specific requirements for advanced research use.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below. This product is hygroscopic (moisture-sensitive). For long-term storage, it is recommended to keep the material desiccated at low temperature. Aliquot contents to minimize repeated freeze-thaw cycles and exposure to ambient conditions.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC/MS)	Conforms
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 3.0%
Biological Activity	Contact for details

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.