Description

5-(n-(n-Biotinyl-ε-Aminocaproyl)-3 -Aminoallyl is a specialized biotinylated derivative designed for advanced biochemical conjugation and labeling applications. This compound is critical for researchers and manufacturers requiring high-affinity, site-specific biotin tagging of biomolecules, enabling downstream detection, purification, and analysis. It is essential for the development of sensitive diagnostic assays, proteomics research, and the production of biotinylated probes and reagents in the pharmaceutical and life sciences industries.

Application

• Bioconjugation & Labeling: Site-specific biotinylation of antibodies, proteins, peptides, and nucleic acids for use in immunoassays (ELISA, Western Blot) and diagnostic kits.
• Affinity Purification: Creation of biotin-tagged ligands for capture and purification using streptavidin/avidin-based resins and columns.
• Proteomics & Biomarker Discovery: Labeling of proteins for pull-down assays, protein-protein interaction studies, and mass spectrometry analysis.
• Diagnostic Reagent Manufacturing: Key intermediate in the synthesis of high-sensitivity detection reagents for clinical and research diagnostics.
• Cell Biology & Imaging: Preparation of biotinylated probes for fluorescence-activated cell sorting (FACS), microscopy, and in situ hybridization.
• Drug Delivery & Targeting: Conjugation to drug molecules or nanoparticles for targeted delivery systems utilizing biotin-receptor interactions.

Basic Information

Item	Detail
Product Name	5-(n-(n-Biotinyl-ε-Aminocaproyl)-3 -Aminoallyl
CAS No.	120964-50-3
Molecular Formula	C26H44N6O5S
Molecular Weight	552.73 g/mol
Synonyms	5-(N-(N-Biotinyl-6-aminocaproyl)-3-aminoallyl); Biotin-XX, SE; Biotin-XX succinimidyl ester; EZ-Link Biotin-XX, SE; 6-((6-((3aS,4S,6aR)-2-Oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)hexanamido)hexanamido)allyl; Biotinylated Aminocaproyl Spacer Arm Reagent; N-Biotinyl-N'-(5-aminopentyl)oxalamide active ester
EINECS	Contact for details

Quality Control

Our 5-(n-(n-Biotinyl-ε-Aminocaproyl)-3 -Aminoallyl is manufactured under strict quality systems suitable for research and in-vitro diagnostic use. Each batch undergoes rigorous analytical testing, including HPLC for purity and spectroscopic identification (NMR, MS), to ensure consistency and performance. Certificates of Analysis (COA) with detailed specifications are provided with every shipment to guarantee traceability and compliance with your project requirements.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC/MS)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 2.0%
Solubility	Soluble in DMSO, DMF; slightly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.