Description

5-((4-Azidophenacyl)Thio)Uridine 5'-Triphosphate is a chemically modified nucleotide triphosphate analog designed for advanced molecular biology and biochemical research. This compound is a critical reagent for photoaffinity labeling and cross-linking experiments, enabling the study of nucleic acid-protein interactions and enzyme mechanisms. It is primarily utilized by researchers in pharmaceutical R&D, biotechnology, and academic institutions focused on structural biology, enzymology, and nucleic acid chemistry.

Application

• Photoaffinity Labeling: Acts as a photoactivatable cross-linker to covalently tag and identify proteins that interact with RNA or DNA.
• Enzyme Mechanism Studies: Used as a substrate analog to probe the active sites and catalytic mechanisms of RNA polymerases, kinases, and other nucleotide-processing enzymes.
• Structural Biology: Facilitates the mapping of binding sites in ribonucleoprotein complexes (RNPs) for structural analysis via techniques like X-ray crystallography or cryo-EM.
• Nucleic Acid Probe Development: Incorporated into oligonucleotides to create light-sensitive probes for detecting specific biomolecular interactions.
• Bioconjugation: Serves as a key intermediate for the synthesis of functionalized nucleotides used in diagnostic assay development and targeted drug delivery systems.

Basic Information

Product Name	5-((4-Azidophenacyl)Thio)Uridine 5'-Triphosphate
CAS No.	120853-61-4
Molecular Formula	C22H24N6O16P3S
Molecular Weight	792.43 g/mol
Synonyms	5-[(4-Azidophenacyl)thio]UTP; 5-((4-Azidophenacyl)thio)uridine-5'-triphosphate; N3-(4-Azidophenacylthio)-UTP; 5-(p-Azidophenacylthio)uridine triphosphate; Azidophenacylthio-UTP; APT-UTP; Photoactivatable UTP; CAS 120853-61-4
EINECS	Contact for details

Quality Control

Our 5-((4-Azidophenacyl)Thio)Uridine 5'-Triphosphate is manufactured under stringent quality protocols to ensure high purity and batch-to-batch consistency for research applications. Each lot is characterized by advanced analytical techniques including HPLC, NMR, and mass spectrometry. A comprehensive Certificate of Analysis (COA) detailing purity, identity, and stability is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a desiccated environment. This compound is strictly light-sensitive (protect from all light exposure) and hygroscopic (moisture-sensitive). For long-term stability, store under inert atmosphere. Allow the vial to equilibrate to room temperature before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 2.0%
Solubility	Soluble in water, DMSO

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.