Description

Polyadenylic Acid-Dodecathymidylic Acid is a synthetic, double-stranded oligonucleotide complex formed from polyadenylic acid and dodecathymidylic acid sequences. This compound is a critical research-grade biochemical tool for studying nucleic acid interactions, hybridization kinetics, and the structural dynamics of DNA-RNA complexes. It is primarily utilized by research institutions and biotechnology companies engaged in molecular biology, biophysical chemistry, and the development of diagnostic assays or therapeutic oligonucleotides.

Application

• Molecular Biology Research: Serves as a model substrate for studying DNA-RNA hybridization, duplex stability, and the mechanisms of nucleic acid-protein interactions.
• Biophysical Studies: Used in spectroscopic analyses (e.g., UV melting, CD spectroscopy) to investigate the thermodynamics and secondary structure of oligonucleotide complexes.
• Diagnostic Assay Development: Acts as a control or calibrator in the development of PCR-based assays, hybridization probes, and other molecular diagnostic platforms.
• Therapeutic Oligonucleotide Research: Provides a reference compound for the design and testing of antisense oligonucleotides, siRNA, and other gene-silencing technologies.
• Enzyme Kinetics: Used as a defined substrate to study the activity, specificity, and inhibition of nucleases, polymerases, and helicases.
• Academic & Pharmaceutical R&D: A fundamental reagent in university and industrial laboratories for foundational research in genetics and drug discovery pipelines.

Basic Information

Product Name	Polyadenylic Acid-Dodecathymidylic Acid
CAS No.	118539-73-4
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	Poly(A)-dT12; Poly(rA)-d(pT)12; Polyadenylate-Dodecathymidylate Complex; Poly A-dT12 Oligonucleotide; dT12-poly(A) Hybrid; Poly(rA)·oligo(dT)12; Polyadenylic Acid·Dodecathymidylic Acid Complex; DNA-RNA Hybrid Oligomer (A12-T12)
EINECS	Contact for details

Quality Control

Our Polyadenylic Acid-Dodecathymidylic Acid is manufactured under controlled conditions to ensure high purity and batch-to-batch consistency, suitable for sensitive research applications. Each lot is analyzed for identity, purity, and duplex integrity. A comprehensive Certificate of Analysis (COA) is provided, detailing specifications such as HPLC purity, absorbance ratios, and melting temperature (Tm). We adhere to stringent internal quality standards to support reliable and reproducible scientific results.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This material is hygroscopic (moisture-sensitive); allow the vial to equilibrate to room temperature before opening to prevent condensation. For long-term stability, store desiccated.

Specification

Item	Specification
Appearance	White to off-white lyophilized powder
Identification (HPLC/UV)	Conforms
Purity (HPLC)	≥ 95%
Absorbance Ratio (A260/A280)	1.8 - 2.0
Duplex Integrity (Melting Curve)	Single, sharp transition
Moisture Content (KF)	≤ 5.0%
Endotoxin (LAL)	< 10 EU/mg

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.