Description

n-Blocked-5'-o-Dmt-2'-o-Tbdms Ced Cytidine Phosphoramidite is a high-purity, chemically modified nucleoside phosphoramidite building block, essential for the automated solid-phase synthesis of oligonucleotides. This compound is critical for ensuring high coupling efficiency and sequence fidelity in the production of research-grade and therapeutic nucleic acids. It is primarily required by biotechnology and pharmaceutical companies engaged in antisense drug development, siRNA research, diagnostic probe synthesis, and other advanced molecular biology applications.

Application

• Oligonucleotide Synthesis: As a key monomer for the automated solid-phase synthesis of DNA and RNA strands containing modified cytidine residues.
• Therapeutic Nucleic Acid R&D: For the production of antisense oligonucleotides (ASOs), small interfering RNA (siRNA), and aptamers for drug discovery and development.
• Diagnostic Probe Manufacturing: Used in creating labeled probes for techniques like FISH (Fluorescence In Situ Hybridization) and microarray analysis.
• PCR Primer and Gene Fragment Synthesis: Enables the incorporation of specific, protected cytidine analogs into primers and synthetic genes.
• Basic Biochemical Research: A vital reagent for academic and industrial research exploring nucleic acid structure, function, and interactions.
• CRISPR Guide RNA Production: For synthesizing chemically modified guide RNA (gRNA) components to enhance stability and specificity in gene-editing applications.

Basic Information

Product Name	n-Blocked-5'-o-Dmt-2'-o-Tbdms Ced Cytidine Phosphoramidite
CAS No.	118380-84-0
Molecular Formula	C46H61N4O8PSi
Molecular Weight	865.06 g/mol
Synonyms	5'-O-(4,4'-Dimethoxytrityl)-2'-O-(tert-butyldimethylsilyl)-N4-benzoyl-3'-O-(2-cyanoethyl-N,N-diisopropylphosphoramidite)cytidine; Ced Phosphoramidite; N4-Benzoyl-5'-O-(4,4'-dimethoxytrityl)-2'-O-[(1,1-dimethylethyl)dimethylsilyl]-3'-O-[2-cyanoethyl(diisopropylamino)phosphino]cytidine; 2'-O-TBDMS-Ced Phosphoramidite; N-Bz-5'-DMT-2'-TBDMS-Cytidine CE Phosphoramidite; RNA Phosphoramidite Monomer; Cytidine Phosphoramidite, N-benzoyl-2'-O-TBDMS protected
EINECS	Contact for details

Quality Control

Our n-Blocked-5'-o-Dmt-2'-o-Tbdms Ced Cytidine Phosphoramidite is manufactured under strict quality systems to ensure the high purity and consistency required for sensitive oligonucleotide synthesis. Each batch undergoes rigorous analytical testing, including HPLC for purity and 31P & 1H NMR for structural confirmation, to meet exacting research and GMP-grade standards. A comprehensive Certificate of Analysis (COA) detailing identity, purity, moisture content, and performance in coupling tests is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere (argon or nitrogen) in a dry, moisture-free environment. This product is hygroscopic (moisture-sensitive) and easily oxidized. Allow the sealed vial to equilibrate to room temperature before opening to prevent condensation.

Specification

Item	Specification
Appearance	White to off-white solid or foam
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 0.5%
Solubility	Clear, colorless solution in anhydrous acetonitrile
Coupling Efficiency	≥ 99.0% (by trityl assay)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.