Description

Cis,Syn-Thymidine Dimer Methyl Phosphoramidite is a high-purity, specialty phosphoramidite monomer essential for the solid-phase synthesis of oligonucleotides. This compound is critical for introducing site-specific modifications, particularly thymine dimers, which are vital for studying DNA damage, repair mechanisms, and developing advanced molecular probes. It is primarily used by research institutions, diagnostic kit manufacturers, and pharmaceutical companies engaged in antisense therapy development, PCR probe synthesis, and foundational genomics research.

Application

• Oligonucleotide Synthesis: Key building block for the automated solid-phase synthesis of DNA and RNA strands containing specific thymine dimer lesions.
• Molecular Biology Research: Used to create probes and primers for studying ultraviolet (UV) light-induced DNA damage and nucleotide excision repair pathways.
• Therapeutic Development: Essential in the research and development of antisense oligonucleotides and other nucleic acid-based therapeutics targeting specific genetic sequences.
• Diagnostic Assays: Manufacture of high-specificity probes for PCR, qPCR, and microarray-based diagnostic tests.
• Bioconjugation & Labeling: Serves as an intermediate for attaching reporter molecules, such as fluorophores or quenchers, to oligonucleotides at precise locations.
• Structural Studies: Synthesis of custom oligonucleotides for X-ray crystallography and NMR spectroscopy to analyze DNA structure and protein-DNA interactions.

Basic Information

Product Name	Cis,Syn-Thymidine Dimer Methyl Phosphoramidite
CAS No.	118187-67-0
Molecular Formula	C25H34N5O12P
Molecular Weight	627.54 g/mol
Synonyms	5'-O-(4,4'-Dimethoxytrityl)-thymidylyl-(3'→5')-thymidine-3'-O-methyl-N,N-diisopropylphosphoramidite; cis,syn-Cyclobutane Thymine Dimer Phosphoramidite; Thymine Dimer Phosphoramidite; cis-syn T<>T Phosphoramidite; DMT-cis,syn-TT Phosphoramidite; [5'-O-(DMT)-2'-deoxythymidylyl-(3'-5')-2'-deoxythymidine] 3'-O-methyl-N,N-diisopropylphosphoramidite; TT Dimer Phosphoramidite
EINECS	Contact for details

Quality Control

Every batch of Cis,Syn-Thymidine Dimer Methyl Phosphoramidite is manufactured under strict quality-controlled conditions. We employ advanced analytical techniques, including HPLC, 31P NMR, and MS, to ensure identity, purity, and performance in oligonucleotide synthesis. Our quality system is designed to meet the rigorous demands of research and pre-clinical development. A comprehensive Certificate of Analysis (COA) detailing purity, moisture content, and functional performance is provided with each shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere (argon or nitrogen) in a desiccated environment. This product is hygroscopic (moisture-sensitive) and easily oxidized. Allow the sealed vial to equilibrate to room temperature before opening to prevent moisture condensation. For long-term stability, store in the original sealed packaging.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms
Identification (31P NMR)	Conforms
Purity (HPLC)	≥ 97.0%
Moisture (Karl Fischer)	≤ 0.5%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.