Description

α-Thioadenosine Triphosphate (CAS NO 117750-47-7) is a chemically modified nucleotide analog where a sulfur atom replaces an oxygen atom in the phosphate chain, creating a hydrolysis-resistant thiophosphate linkage. This structural modification is critical for its role as a potent and stable substrate or inhibitor for ATP-dependent enzymes, particularly kinases and polymerases. It is an essential biochemical tool for researchers in pharmaceutical discovery, enzymology, and molecular biology, enabling detailed mechanistic studies and high-throughput screening assays.

Application

• Enzyme Kinetics & Mechanistic Studies: Used as a non-hydrolyzable or slow-turnover ATP analog to study the mechanisms of ATP-binding enzymes like kinases, ATPases, and helicases.
• High-Throughput Screening (HTS): Serves as a key substrate in assay development for identifying and characterizing kinase inhibitors in drug discovery pipelines.
• Biochemical Probe Development: Utilized in the synthesis of affinity probes or photoaffinity labels for target identification and protein profiling.
• Nucleic Acid Research: Acts as a substrate for RNA polymerases in transcription studies or for the enzymatic synthesis of modified RNA strands.
• Structural Biology: Employed in X-ray crystallography and NMR studies to capture and determine the structures of enzyme-substrate complexes.
• Diagnostic Assay Development: Incorporated into enzymatic detection systems for clinical diagnostics and biosensor applications.

Basic Information

Product Name	α-Thioadenosine Triphosphate
CAS No.	117750-47-7
Molecular Formula	C10H16N5O12P3S
Molecular Weight	523.31 g/mol
Synonyms	Adenosine 5'-(γ-thio)triphosphate; ATPγS; Adenosine 5'-(3-thiotriphosphate); γ-Thio-ATP; Adenosine-5'-triphosphorothioate; 5'-Adenylyl (γ-thio)diphosphonate; Thiophosphoryl adenosine triphosphate; Sp-isomer of ATPαS (common reference)
EINECS	Contact for details

Quality Control

Our α-Thioadenosine Triphosphate is manufactured under strict quality systems to ensure batch-to-batch consistency and reliability for sensitive research applications. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and impurity profiles. We adhere to ISO 9001 quality management principles, and our analytical methods, including HPLC and NMR, are validated to provide accurate specifications for the research and pharmaceutical markets.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a desiccated environment. This product is hygroscopic (moisture-sensitive) and should be allowed to equilibrate to room temperature in a dry atmosphere before opening to prevent condensation and hydrolysis. For long-term stability, store under inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white powder or lyophilized solid
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95% (Anhydrous basis)
Water Content (KF)	≤ 3.0%
Biological Activity (Enzymatic Assay)	≥ 90% of theoretical activity
Endotoxin (LAL)	< 10 EU/mg (if specified)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.