Description

5'-o-Triphosphoryl-8-Azidoadenylyl-(2'-5')-8-Azidoadenylyl-(2'-5')-8-Azidoadenosine is a chemically modified trinucleotide analog, specifically designed as a photoactivatable crosslinking agent. This compound is critical for advanced biochemical research, enabling the study of RNA-protein interactions, enzyme mechanisms, and nucleic acid structure through covalent bond formation upon UV irradiation. It is primarily utilized by research institutions, pharmaceutical R&D departments, and biotechnology companies focused on nucleic acid chemistry, photochemistry, and molecular probe development.

Application

• Photoaffinity Labeling: As a key reagent for covalently trapping and identifying RNA-binding proteins and other macromolecular interactions.
• Mechanistic Enzymology: Used to probe the active sites and catalytic mechanisms of nucleotidyltransferases, polymerases, and other nucleic acid-modifying enzymes.
• Structural Biology Studies: Employed in crosslinking experiments to map RNA tertiary structure and RNA-protein complex architecture.
• Probe Synthesis: Serves as a building block for creating advanced diagnostic or therapeutic probes that target specific RNA sequences.
• Bioconjugation: Facilitates the site-specific attachment of nucleic acid motifs to surfaces, particles, or other biomolecules via the azide group.
• Antiviral Research: Potential use in studying the 2'-5'-oligoadenylate synthetase/RNase L pathway, which is part of the innate immune response to viral infection.

Basic Information

Item	Detail
Product Name	5'-o-Triphosphoryl-8-Azidoadenylyl-(2'-5')-8-Azidoadenylyl-(2'-5')-8-Azidoadenosine
CAS No.	117146-01-7
Molecular Formula	C30H34N18O25P5
Molecular Weight	1268.58 g/mol
Synonyms	8-Azido-2',5'-triadenylic acid; pppA2'p5'(8-N3A)2'p5'(8-N3A); 5'-Triphosphoryl-8-azido-2',5'-triadenylate; 8-Azido-2-5A triphosphate; 2'-5' ppp(A2'p)2A(8N3); Photoactivatable 2-5A trimer triphosphate; 8-N3-2-5A triphosphate; 2',5'-Oligoadenylate triphosphate derivative
EINECS	Contact for details

Quality Control

Every batch of our 5'-o-Triphosphoryl-8-Azidoadenylyl-(2'-5')-8-Azidoadenylyl-(2'-5')-8-Azidoadenosine is synthesized and purified under controlled conditions to ensure the highest standards for research applications. Quality is verified through comprehensive analytical techniques including HPLC, UV-Vis spectroscopy, and mass spectrometry to confirm identity, purity, and functional integrity. A detailed Certificate of Analysis (COA) documenting purity, absorbance characteristics, and stability is provided with each shipment to support your critical research and development work.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a desiccated environment. This compound is hygroscopic (moisture-sensitive) and light-sensitive. For long-term stability, aliquoting under an inert atmosphere is recommended to prevent degradation. Allow the vial to equilibrate to room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms
Identification (MS)	Conforms
Purity (HPLC)	≥ 95%
UV-Vis Spectrum	Characteristic of 8-azidoadenosine derivatives
Water Content (KF)	≤ 3.0%
Solubility	Soluble in aqueous buffers, DMSO

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.